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Molecular Signatures and Functional Analysis of Secondary Hair Follicles in Cashmere Goat During Annual Cashmere Growth

ABSTRACT: Secondary hair follicles (SHFs) produce the cashmere of goats. In the present study, SHF clusters were isolated under the microscope by cutting out the epidermis and removing the connective tissues. RNA sequencing was performed on the collected SHFs. Through genome-wide transcriptomic analysis, we discovered and characterized differentially expressed molecular signatures across the annual growth cycle of cashmere and demonstrated their differential expression features and importance in cashmere growth.

ORGANISM(S): Capra hircus

PROVIDER: GSE221472 | GEO | 2023/01/31

REPOSITORIES: GEO

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Discovery and Functional Analysis of Secondary Hair Follicle miRNAs during Annual Cashmere Growth

Project description:Secondary hair follicles (SHF) produce the thermoregulatory cashmere in goat. MiRNAs were reported playing indispensable roles in hair follicle formation and growth. However, most studies examining miRNAs related to cashmere have been performed on goat skin. It still remains obscure that which miRNAs are highly expressed in SHFs or how miRNAs affect the cashmere growth. In the present study, we isolated the SHFs under dissecting microscope and analyzed the miRNA signatures during annual cashmere growth. Small RNA sequencing followed by genome-wide expression analysis reveals that early anagen is a crucial phase for miRNA regulation on the cashmere growth, which was uncovered by two predominant groups of miRNAs. Although they exhibit opposite expression patterns, both groups demonstrated sharp changes of expression when transit from early anagen to mid-anagen. In addition, we identified 96 miRNA signatures that differentially expressed between different phases among 376 miRNAs. Functional analysis of the predicted target genes for highly expressed or differentially expressed miRNAs indicated that these miRNAs were involved in signal pathways associated with SHF development, regeneration and regression. Furthermore, miR-143-3p is preferentially expressed in SHFs and Itga6 was identified as one of targets. The dual-luciferase and in situ hybridization assay demonstrated that miR-143-3p directly repressed the expression of Itga6, suggesting a possible novel role for miR-143-3p in the cashmere growth.

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