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Single-cell sequencing reveals differential cell types in skin tissues of Liaoning Cashmere Goats and key genes related potentially to the fineness of cashmere fiber

ABSTRACT: we used single-cell RNA sequencing (scRNA-seq) and computational models to identify 13 skin cell types in Liaoning Cashmere Goats. We also analyzed the molecular changes by Cell Trajectory Analysis in the development process and revealed the maturation process in gene expression profile in Liaoning Cashmere Goats. Weighted gene co-expression network analysis (WGCNA) explored hub genes in cell clusters related to cashmere formation. Secondary hair follicle dermal papilla cells (SDPCs) play an important role in the growth and density of cashmere. ACTA2, a marker gene of SDPCs, was selected for immunofluorescence (IF) and western blot (WB) verification. Our results indicate that ACTA2 is mainly expressed in SDPCs, and WB results showed different expression levels. COL1A1 is a highly expressed gene in SDPCs, which was verified by IF and WB. We then selected CXCL8 of SDPCs to verify, and prove the differential expression in the coarse type and the fine type of Liaoning Cashmere Goats. Therefore, CXCL8 gene may regulate cashmere fineness. These genes may be involved in regulating the fineness of cashmere in goat secondary hair follicle dermal papilla cells, our research will provide new insights into the mechanism of cashmere growth and cashmere fineness regulation by cells.

ORGANISM(S): Capra hircus

PROVIDER: GSE182474 | GEO | 2021/08/25

REPOSITORIES: GEO

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A single-cell transcriptome atlas during Cashmere goat hair follicle morphogenesis

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2020-10-18 | GSE144351 | GEO

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A Microarray-Based Analysis Reveals That A Short Photoperiod Promotes Hair Growth in the Arbas Cashmere Goat

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2015-11-11 | E-GEOD-74860 | biostudies-arrayexpress

A Microarray-Based Analysis Reveals That A Short Photoperiod Promotes Hair Growth in the Arbas Cashmere Goat

2015-11-11 | GSE74860 | GEO

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Global profiling of human hair follicle scalp dermal papilla cells using Affymetrix microarrays

2013-10-20 | GSE44765 | GEO

Comparative gene expression analysis of 2 subpopulations of dermal papilla cells.

Project description:Different types of hair follicles can be found in the skin of mice. It is believed that the signals that control hair follicle differentiation arise from cells in a structure called the dermal papilla. Understanding the nature of those signals is of interest for the biology of the normal tissue. We have developed a technique for isolation of dermal cells by enzymatic digestion of intact skin. We have identified two subpopulations of cells that can be separated by FACS. The Sox2-positive CD133-positive cells are found exclusively in the dermal papillae of guard/awl/auchene hairs, while Sox2-negative, CD133-positive cells are found in the other hair follicle types. We compared these populations with unfractionated dermal cells. We isolated the following 3 populations of cells from the back skin of neonatal mice (P2) by Flow Cytometry: 1) GFP-CD133- Total dermal cells 2) GFP-CD133+ Dermal Papilla cells 3) GFP+CD133+ Dermal Papilla cells The yield is approximately 50,000 cells of each population.

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Global profiling of human hair follicle scalp dermal papilla cells using Affymetrix microarrays

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2013-10-20 | E-GEOD-44765 | biostudies-arrayexpress

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