Next Generation Sequencing of Transcriptomes from Murine Articular Chondrocytes Treated with IL-1 [Bulk RNA-seq]
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ABSTRACT: Murine articular chondrocytes were isolated as previously described (Gosset et al., 2008) with modifications. Briefly, articular cartilages were dissected from femoral heads of 3-week-old wild-type C57/BL6J mice and digested for 4-6 hours in 0.5 mg/mL collagenase P (Roche, # 11249002001) in high-glucose DMEM (Thermo Fisher Scientific, #10569010) supplemented with 1% penicillin/streptomycin. Following digestion, cells were collected and seeded at a density of 50 x 104 cells/well in 12-well plates. The next day, cells were treated with IL-1 (1 ng/mL) (R&D Systems, #201-LB-005) for 24 hours. Total RNA were then extracted from cell cultures and assayed for RNA sequencing.
ORGANISM(S): Mus musculus
PROVIDER: GSE221483 | GEO | 2023/02/06
REPOSITORIES: GEO
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