Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Characterisation of VDR signaling in prostate cancer health disparities (RNA-Seq)

ABSTRACT: To investigate the effect of 1,25(OH)2D3 activation on RNA expression in prostate cell lines derived from European Americans and African Americans

ORGANISM(S): Homo sapiens

PROVIDER: GSE221690 | GEO | 2023/01/16

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Characterisation of VDR signaling in prostate cancer health disparities (ATAC-Seq)

Project description:To investigate the effect of 1,25(OH)2D3 activation on chromatin accessabilty in prostate cell lines derived from European Americans and African Americans

2023-01-16 | GSE221708 | GEO

Characterisation of VDR signaling in prostate cancer health disparities (ChIP-Seq)

Project description:To investigate the effect of 1,25(OH)2D3 activation on VDR genomic binding in prostate cell lines derived from European Americans and African Americans

2023-01-16 | GSE221826 | GEO

Characterisation of VDR signaling in prostate cancer health disparities (small RNA-Seq)

Project description:To investigate the effect of 1,25(OH)2D3 activation on small RNA expression in prostate cell lines derived from European Americans and African Americans

2023-01-31 | GSE223249 | GEO

Characterisation of VDR signaling in prostate cancer health disparities (RNA-Seq; BAZ1A conditional)

Project description:To investigate the effect of 1,25(OH)2D3 activation on RNA expression in prostate cell lines derived from European Americans and African Americans

2023-01-31 | GSE223405 | GEO

Vitamin D receptor (VDR) target genes in THP-1 monocytic leucemia cells

Project description:The biologically active form of vitamin D, 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), is a direct regulator of gene transcription, since it is the only high affinity natural ligand of the transcription factor vitamin D receptor (VDR). Transcriptome-wide analysis of THP-1 human monocyte-like cells had indicated more than 600 genes to be significantly (p < 0.05) regulated after a 4 h stimulation with 1,25(OH)2D3. In this study, we screened of the list of primary vitamin D targets for genes encoding for transcriptional regulators and selected those of the activating transcription factor NFE2 and the transcriptional repressor BCL6. Both genes are under the control of two VDR loci and are the only 1,25(OH)2D3 targets within their respective chromosomal domain. However, NFE2 mRNA was rapidly up-regulated, while the increase of BCL6 expression showed a slower rise. After 24 h incubation of THP-1 cells with 1,25(OH)2D3 more than 1,500 genes responded significantly (p < 0.001), of which 132 where more than 2-fold induced. Public chromatin immunoprecipitation-sequencing datasets suggested that the majority of these genes could be targets of NFE2 or BCL6. In time course experiments we displayed for representative gene examples the specific delayed response of secondary 1,25(OH)2D3 targets and confirmed for the respective chromosomal domains the genomic binding of NFE2, BCL6 and VDR. In conclusion, our study indicated that the physiological response of monocytes to 1,25(OH)2D3 involves the action of NFE2 and BCL6. THP-1 cells were treated 24 h either with 0.1% ethanol (vehicle, control) or 1?,25(OH)2D3 (1,25D)

2015-04-26 | E-GEOD-60102 | biostudies-arrayexpress

Vitamin D receptor (VDR) target genes in THP-1 monocytic leucemia cells

2015-04-26 | GSE60102 | GEO

VDR ChIP-seq in human monocytic THP-1 cells

Project description:ChIP-seq for VDR in THP-1 cells after stimulation with the the natural VDR ligand 1,25-dihydroxyvitamin D3 (1,25(OH)2D3)

2017-07-18 | GSE89431 | GEO

Vitamin D receptor (VDR) ChIP-Seq on human colonic organoids stimulated with 1,25(OH)2D3

Project description:Genome-wide VDR chromatin binding sites in colonic organoids stimulated with 1,25(OH)2D3 for 2h.

2024-07-01 | GSE206176 | GEO

Differential and overlapping effects of 20.23(OH)2D3 and 1,25(OH)2D3 on gene expression in human epidermal keratinocytes

Project description:Neonatal keratinocytes from African American donors of passage 2 or 3 were treated with 20,23(OH)2D3, 1,25(OH)2D3 or 0.1% ethanol (control) for 6 and 24 hours. The cells were harvested separately, RNA isolated and submitted for microarray analysis at the Molecular Resources Center at the UTHSC.

2018-07-19 | GSE117351 | GEO

mRNA-seq in VDR knockout and control THP-1 cells

Project description:The vitamin D receptor (VDR) has been knocked out in monocytic THP-1 cells after stimulation of ko and control cells with the the natural VDR ligand 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), quadruplicate mRNA-seq has been performed

2021-09-06 | GSE157514 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data