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LEC1::GR induction: Establishing the network of seed gene expression and analysis of its biodiversity

ABSTRACT: lec1::gr induction - Identification of LEC1 target genes - Induction of 14 days old LEC1::GR seedlings , different treatments (EtOH, DEX, DEX+ABA, induction) for 8 hours

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE22173 | GEO | 2011/08/31

SECONDARY ACCESSION(S): PRJNA127683

REPOSITORIES: GEO

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LEC1::GR induction: Establishing the network of seed gene expression and analysis of its biodiversity

Project description:lec1::gr induction - Identification of LEC1 target genes - Induction of 14 days old LEC1::GR seedlings , different treatments (EtOH, DEX, DEX+ABA, induction) for 8 hours 6 dye-swap - CATMA arrays

2011-08-30 | E-GEOD-22173 | biostudies-arrayexpress

Wild type Lec1 transgene induction by DEX: Establishing the network of seed gene expression and analysis of its biodiversity (wt dex induction)

Project description:wt dex induction - Identification of LEC1 target genes - Induction of 14 days old WT seedlings , different treatments ( EtOH, DEX induction) for 8 hours

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wt dex induction-Establishing the network of seed gene expression and analysis of its biodiversity

Project description:tri33-lec1 - wt dex induction - Identification of LEC1 target genes - Induction of 14 days old WT seedlings , different treatments ( EtOH, DEX induction) for 8 hours

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Wild type Lec1 transgene induction by DEX: Establishing the network of seed gene expression and analysis of its biodiversity (wt dex induction)

Project description:wt dex induction - Identification of LEC1 target genes - Induction of 14 days old WT seedlings , different treatments ( EtOH, DEX induction) for 8 hours 2 dye-swap - CATMA arrays

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wt dex induction-Establishing the network of seed gene expression and analysis of its biodiversity

Project description:tri33-lec1 - wt dex induction - Identification of LEC1 target genes - Induction of 14 days old WT seedlings , different treatments ( EtOH, DEX induction) for 8 hours 2 dye-swap - treated vs untreated comparison

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Identification of LEC1 target genes

Project description:The transcription factor (TF) LEAFY COTYLEDON1 (LEC1) acts as an essential regulator of Arabidopsis embryogenesis and seed development. It controls aspects of early embryogenesis such as cotyledon identity and suspensor morphology, as well as seed maturation processes such as storage compound accumulation, acquisition of desiccation tolerance and dormancy. To identify downstream components of the LEC1 regulon, dexamethasone-regulated expression of LEC1 and ChIP/chip were applied and revealed the enrichment of phytohormone- as well as elongation-related genes among LEC1 target genes. Two weeks old 35S::LEC1::GR seedlings were treated with DEX or ethanol for 24 hours before cross-linking and chromatin isolation. Four biological replicates were performed, using an ethanol-treated sample as control (LEC1_EtOH versus LEC1_DEX). After data normalization, statistical analysis and exclusion of putative false negatives, a number of 356 target promoter regions were identified.

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GR accelerates, but is largely dispensable for, adipogenesis

Project description:Dexamethasone (DEX), a synthetic ligand for glucocorticoid receptor (GR), is routinely used to stimulate adipogenesis in culture. GR-depleted preadipocytes show adipogenesis defects one week after induction of differentiation. However, it has remained unclear whether GR is required for adipogenesis in vivo. By deleting GR in precursors of brown adipocytes, we found unexpectedly that GR is dispensable for brown adipose tissue development in mice. In culture, GR-deficient primary or immortalized white and brown preadipocytes showed severely delayed adipogenesis one week after induction of differentiation. However, when differentiation was extended to 3 weeks, GR-deficient preadipocytes showed similar levels of adipogenesis marker expression and lipid accumulation as the wild type cells, indicating that DEX-bound GR accelerates, but is dispensable for, adipogenesis. Consistently, DEX accelerates, but is dispensable for, adipogenesis in culture. We show that DEX-bound GR accelerates adipogenesis by directly promoting the expression of adipogenic transcription factors C/EBPb, C/EBPd, C/EBPa, KLF5, KLF9 and PPARg in the early phase of differentiation. Mechanistically, DEX-bound GR recruits histone H3K27 acetyltransferase CBP to promote activation of C/EBPb-primed enhancers of adipogenic genes. These results clarify the role of GR in adipogenesis in vivo and demonstrate that DEX-mediated activation of GR accelerates, but is dispensable for, adipogenesis.

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tt1-gr-TT1 gene network

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Essential functions of the Glucocorticoid Receptor require direct DNA binding [RNA-seq]

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IP of endogenous SUMO2/3 in HEK293 cells

Project description:Aim of the experiments was to identify endogenously SUMO2/3 modified proteins in HEK293 cells. Cells were treated with dexamethasone (dex) or vehicle (etoh) and with heatshock (+43C, HS) or normal growth temperature (+37C, non-HS). Cells were induced with tetracycline to express BirA*-GR (glucocorticoid receptor) to express GR at sufficient levels to assess effect of GR activation on protein SUMOylation.

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