Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Cpeb1b-mediated cytoplasmic polyadenylation of shha mRNA modulates zebrafish definitive hematopoiesis [RNAseq_cpeb1b_ribominus]

ABSTRACT: Transcriptomics analysis of gene expression in the low dose cpeb1b MO injected wt and cpeb1b mutant zebrafish embryos

ORGANISM(S): Danio rerio

PROVIDER: GSE222074 | GEO | 2023/02/07

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Cpeb1b-mediated cytoplasmic polyadenylation of shha mRNA modulates zebrafish definitive hematopoiesis [RIBO-Seq]

Project description:Ribosome profiling RNA sequencing in the low dose cpeb1b MO injected wt and cpeb1b mutant zebrafish embryos

2023-02-07 | GSE222154 | GEO

Cpeb1b-mediated cytoplasmic polyadenylation of shha mRNA modulates zebrafish definitive hematopoiesis

Project description:During vertebrate embryogenesis, hematopoietic stem and progenitor cell (HSPC) production through endothelial-to-hematopoietic transition requires suitable developmental signals, but how these signals are accurately regulated remains incompletely understood. Cytoplasmic polyadenylation, which is one of the posttranscriptional regulations, plays a crucial role in RNA metabolism. Here, we report that Cpeb1b-mediated cytoplasmic polyadenylation is important for HSPC specification by translational control of Hedgehog (Hh) signaling during zebrafish early development. Cpeb1b is highly expressed in notochord and its deficiency results in defective HSPC production. Mechanistically, Cpeb1b regulates hemogenic endothelium specification by the Hedgehog–Vegf–Notch axis. We demonstrate that the cytoplasmic polyadenylation element motif-dependent interaction between Cpeb1b and shha messenger RNA (mRNA) in the liquid-like condensates, which are induced by Pabpc1b phase separation, is required for cytoplasmic polyadenylation of shha mRNA. Intriguingly, the cytoplasmic polyadenylation regulates translation but not stability of shha mRNA, which further enhances the Shha protein level and Hh signal transduction. Taken together, our findings uncover the role of Cpeb1b-mediated cytoplasmic polyadenylation in HSPC development and provide insights into how posttranscriptional regulation can direct developmental signals with high fidelity to translate them into cell fate transition.

2023-02-06 | GSE207906 | GEO

Cpeb1b-mediated cytoplasmic polyadenylation of shha mRNA modulates zebrafish definitive hematopoiesis

Project description:Cpeb1b-mediated cytoplasmic polyadenylation of shha mRNA modulates zebrafish definitive hematopoiesis

| PRJNA857656 | ENA

Cpeb1b-mediated cytoplasmic polyadenylation of shha mRNA modulates zebrafish definitive hematopoiesis [RNAseq_cpeb1b_ribominus]

Project description:Cpeb1b-mediated cytoplasmic polyadenylation of shha mRNA modulates zebrafish definitive hematopoiesis [RNAseq_cpeb1b_ribominus]

| PRJNA917598 | ENA

Cpeb1b-mediated cytoplasmic polyadenylation of shha mRNA modulates zebrafish definitive hematopoiesis [RIBO-Seq]

Project description:Cpeb1b-mediated cytoplasmic polyadenylation of shha mRNA modulates zebrafish definitive hematopoiesis [RIBO-Seq]

| PRJNA918384 | ENA

Zebrafish embryos: Control MO vs. Lin28A MO vs. Lin28B MO injected

Project description:miRNA profiling of zebrafish embryos comparing control MO with Lin28A MO or Lin28B MO injected embryos at 5hpf

2013-11-19 | GSE52492 | GEO

SMN MO, Gemin2 MO knockdown vs Control MO injected

Project description:Transcriptiome profiling of SMN MO injected, Gemin2 MO injected zebrafish embryos vs Control MO injected

2013-12-05 | GSE47001 | GEO

Zebrafish (Danio rerio) 72hpf, WT control vs. MO-Prpf31 injected

Project description:Transcriptional profiling performed from total eye RNA extracts of wildtype control fishes versus Prpf31 morpholino injected larvae (at ~72hpf) two-condition experiment: wildtype zebrafish versus MO-Prpf31 injected zebrafish eye RNA; 6 replicates each (extraction from 6 pools (~200 eyes each) of controls and 6 pools MO-Prpf31 (~200 eyes each))

2010-05-06 | E-GEOD-20424 | biostudies-arrayexpress

Zebrafish embryos: Control MO vs. Lin28A MO vs. Lin28B MO injected

Project description:miRNA profiling of zebrafish embryos comparing control MO with Lin28A MO or Lin28B MO injected embryos at 5hpf One-condition experiment

2013-11-19 | E-GEOD-52492 | biostudies-arrayexpress

Development of gene expression signatures after knocking down of miR-132 in zebrafish

Project description:To further development of the gene expression, we have employed whole genome microarray expression profiling as a discovery platform to identify genes that are regulated in miR-132 knockdown zebrafish. Zebrafish larvae were injected with Control MO or miR-132 MO at one-cell stage. Then the upregulated genes are combined to the miRNA prediction alagorithm. 21 genes were picked up and verifed by luciferase assay. The total RNA was extracted from 3 dpf zebrafish injected with 8 ng control or miR-132 MO MO. The RNA was applied for the microarray gene proliling

2016-08-09 | E-GEOD-85291 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data