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Development of gene expression signatures after knocking down of miR-132 in zebrafish

ABSTRACT: To further development of the gene expression, we have employed whole genome microarray expression profiling as a discovery platform to identify genes that are regulated in miR-132 knockdown zebrafish. Zebrafish larvae were injected with Control MO or miR-132 MO at one-cell stage. Then the upregulated genes are combined to the miRNA prediction alagorithm. 21 genes were picked up and verifed by luciferase assay. The total RNA was extracted from 3 dpf zebrafish injected with 8 ng control or miR-132 MO MO. The RNA was applied for the microarray gene proliling

ORGANISM(S): Danio rerio

SUBMITTER: Jiu-lin Du

PROVIDER: E-GEOD-85291 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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