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Characterization and comparative profiling of microRNA transcriptomes in bighead carp and silver carp

ABSTRACT: We applied high throughput sequencing technology to identify microRNA genes in bighead carp and silver carp. We identified 167 conserved miRNAs in bighead carp and 166 in silver carp. By two computational stragegies, we obtained 39 novel miRNAs in bighead carp and 54 in silver carp, for which, no homologs were found in other species. Several miRNA* sequences were found in our dataset as well, some particular ones might have gene regulation function. Gain and loss of family members were observed in several miRNA families, which partially reflected the fate of miRNA gene duplicates.

ORGANISM(S): Hypophthalmichthys nobilis Hypophthalmichthys molitrix

PROVIDER: GSE22232 | GEO | 2010/09/01

SECONDARY ACCESSION(S): PRJNA128843

REPOSITORIES: GEO

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Characterization and comparative profiling of microRNA transcriptomes in bighead carp and silver carp

Project description:We applied high throughput sequencing technology to identify microRNA genes in bighead carp and silver carp. We identified 167 conserved miRNAs in bighead carp and 166 in silver carp. By two computational stragegies, we obtained 39 novel miRNAs in bighead carp and 54 in silver carp, for which, no homologs were found in other species. Several miRNA* sequences were found in our dataset as well, some particular ones might have gene regulation function. Gain and loss of family members were observed in several miRNA families, which partially reflected the fate of miRNA gene duplicates. Total RNA of juvenile bighead carp and silver carp were sequenced on one Solexa lane, respectively.

2010-09-01 | E-GEOD-22232 | biostudies-arrayexpress

Hypophthalmichthys molitrix cultivar:Silver carp

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Silver carp (Hypophthalmichthys molitrix) dorsal muscle LC-MSMS data

Project description:China produces more than 77.9% of the world's production of silver carp in 2020 with the production of 3812.9 kiloton.3 The high consumption of silver carp in China is mainly by using its edible muscles for manufacture of surimi-based seafoods or other muscle foods, which may contribute high quality protein resource and other valuable nutrients in human diets. This project is to understanding the muscle composition of slaughtered fish skeletal muscle use the proteomics methods. The proteomics was performed based on the improved DDA experiment with extensive fractionation and prolonged separation of peptides, and protein searching database was informed by the Iso-seq transcriptomics.

2024-01-26 | PXD043702 | Pride

Characterization and comparative profiling of microRNA transcriptomes in bighead carp and silver carp

Project description:Characterization and comparative profiling of microRNA transcriptomes in bighead carp and silver carp

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Transcriptome analysis of Bacillus cereus B3711 during silver ion stress condition

Project description:We have employed a whole genome microarray for gene expression profilling to understand the global view of the cellular mechanism and metabolic response of B. cereus B3711 exposed to 1 mM silver nitrate. RNA was extracted from B. cereus grown under silver treated and untreated set of conditions in duplicates at two different time points (30 & 60 min). Comparing the transcript profile of the control and treated cells, several differentially expressed genes were identified. These includes genes involved in various metabolic pathways, such as arginine and alanine metabolism, membrane transport system and alternate respiratory proteins such as arsenate reductase, stress related proteins, proteins of transcriptional regulators, hypothetical proteins and protein of two-component systems.

2010-12-14 | GSE26043 | GEO

Mixed baits reduce the oxidative stress of common carp (Cyprinus carpio): Based on microRNA sequencing

Project description:Purpose:In order to elucidate the molecular mechanism of the effects of different diets on the antioxidant capacity of fish, three groups of adult common carp (Cyprinus carpio) (initial weight 517.8 ±50 g) were fed with earthworm, earthworm + duckweed and duckweed, respectively, which were performed by miRNA sequencing. Methods:MiRNA profiles of common carp fed with earthworm, earthworm + duckweed and duckweed were generated by deep sequencing, in duplicate, using Illumina Hiseq 2500 platform. qRT–PCR validation was performed using SYBR Green assays Results: We built microRNA libraries by using total RNA and contained filtered 20,868,000, 18,984,242, 22,300,487, 19,538,683, 17,110,676 and 14,747,090 high-quality reads that were obtained from sample A1, A2, M1, M2, P1 and P2 respectively.In total, we discovered 236 known carp miRNAs (225, 225, 222, 227, 228 and 219 in A1, A2, M1, M2, P1and P2, respectively).For the known miRNA, 9 differentially expressed miRNAs (DEM) were found between A and M and 8 known miRNAs differentially expressed between P and M, and 11 of these were validated with qRT–PCR. The expression level of 11 known microRNAs via miRNA-seq had highly similar with the relative expression level of 11 known microRNAs via qRT-PCR. Conclusions: In present study, we demonstrated the transcriptional profiles, identification of 5 differentially expressed miRNAs be reported related to oxidative stress, including miR-137-3p, miR-143-3p, miR-146a-5p, miR-21-5p and miR-125b-5p. For omnivorous fish, feed a single bait may might cause oxidative stress inordinately.

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Transcriptomic analysis of silver-resistant Saccharomyces cerevisiae mutant obtained by evolutionary engineering

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Transcriptome analysis of Bacillus cereus B3711 during silver ion stress condition

2010-12-14 | E-GEOD-26043 | biostudies-arrayexpress

Identification of miRNA from Cyprinus carpio by high-throughput sequencing and bioinformatics analysis

Project description:Common carp is one of the main commercial fishes captured and cultured worldwide. Although common carp genome is not finished yet, this study provides a first large scale cloning and characterization of common carp miRNAs and their potential targets. These miRNAs add to the growing database of new miRNA and lay the foundation for further understanding of miRNA function in gene regulation of common carp.

2012-07-16 | GSE35131 | GEO

Waterborne exposure of adult zebrafish to silver nanoparticles and ionic silver results in silver accumulation and effects at cellular and molecular levels

Project description:Effects of silver nanoparticles (Ag NPs) on freshwater species have been reported in several studies, but there is not information on the potential long-term consequences of a previous exposure. In this work, we investigated the long-term effects of maltose-coated Ag NPs (20 nm) and of ionic silver (10 µg/L) after 21 days of exposure and at 6 months post-exposure (mpe) in adult zebrafish. Exposure resulted in significant silver accumulation in the whole body of fish exposed to ionic silver, but not in those exposed to Ag NPs. However, autometallography revealed metal accumulation in the liver and intestine of fish treated with the two silver forms and especially in the intestine of fish exposed to Ag NPs. X-ray microanalysis showed the presence of silver in gills, liver and intestine and of Ag NPs in gill and liver cells. Inflammation and hyperplasia were evident in the gills after both treatments and these histopathological conditions remained at 6 mpe. According to the hepatic transcriptome analysis, at 3 days ionic silver regulated a larger number of transcripts (410) than Ag NPs (129), while at 21 days Ag NPs provoked a stronger effect (799 vs 165 regulated sequences). Gene ontology terms such as “metabolic processes” and “response to stimulus” appeared enriched after all treatments, while “immune system” or “reproductive processes” were specifically enriched after the exposure to Ag NPs. This suggests that the toxicity of Ag NPs may not be solely related to the release of Ag ions, but also to the NP form. No evident effects were found on protein oxidation or on hepatocyte lysosomal membrane stability during exposure, but effects recorded on liver lysosomes and persistent damage on gill tissue at 6 mpe could indicate potential for long-term effects in exposed fish.

2018-06-12 | GSE89653 | GEO

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