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Neutrophil metalloproteinase driven spleen architecture breakdown hampers plasma cell generation and infection control of trypanosomiasis.

ABSTRACT: Recent blood transcriptomic analysis of rhodesiense sleeping sickness patients has revealed that neutrophil signature genes and activation markers constitute the top indicators of trypanosomiasis-associated inflammation. Here, we used single cell RNA sequencing (scRNA-seq) to analyze the diversity of neutrophils in the spleen. We show that Trypanosoma brucei infection results in expansion and differentiation of four splenic neutrophil subpopulations, including Mki67+Birc5+Gfi1+Cebpe+ proliferation-competent precursors, two intermediate immature subpopulations and Cebpb+Spi1+Irf7+Mcl1+Csf3r+ inflammation reprogrammed mature neutrophils. Transcriptomic scRNA-seq profiling identified the largest immature subpopulation by Mmp8/9 positive tertiary granule markers.

ORGANISM(S): Mus musculus

PROVIDER: GSE222784 | GEO | 2023/08/10

REPOSITORIES: GEO

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Neutrophil metalloproteinase driven spleen architecture breakdown hampers plasma cell generation and infection control of trypanosomiasis.

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