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Enrichment of sgRNA in a genome-wide CRISPR-cas9 knockout screening assay in HSV-1 infected the human amnionic WISH cells models.


ABSTRACT: The Human CRISPR Knockout Pooled Library (GeCKO v2, 1000000048, Addgene) was transferred into WISH cells. Then,half of these cells were infected with HSV-1 for 30 hours, and the remaining lived infected cells and uninfected cells were collected for sgRNA sequencing and enrichment analysis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE223085 | GEO | 2024/06/01

REPOSITORIES: GEO

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