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Optimized minimal genome-wide human sgRNA library

ABSTRACT: To minimize the human genome-wide CRISPR/Cas9 library size, we established H-mLib which recruited a novel sgRNA design method and applied with dual plasmid based strategy. The performance of the H-mLib was benchmarked to other CRISPR libraries in a proliferation screening conducted in K562 cells. We also identified human core essential genes and cell-type specific essentials genes in K562 and Jurkat cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE223086 | GEO | 2023/07/18

REPOSITORIES: GEO

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