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Circadian transcriptomic analysis of mouse liver during a time restricted feeding

ABSTRACT: Determination, using microarrays, of the mouse hepatic global programme of gene expression variations around the clock when animals had food access restricted to the active periode (ZT12 to ZT24) after a 11 days training periode The unique functional versatility of the liver is paramount for the organism homeostasis. Both liver development and adult life are controlled by tightly regulated transcription factor networks, among which nuclear receptors (NRs) regulate essential functions of parenchymal and nonparenchymal cells. Acting as transcription factors sensitive to extracellular cues such as steroidal hormones, lipid metabolites, xenobiotics… and modulated by intracellular signaling pathways, NRs orchestrate many aspects of hepatic physiology. While liver functional zonation and adaptability to fluctuating conditions are known to rely on a sophisticated cellular architecture, the exact functions of NRs in liver cell types remain poorly characterized. As a first step toward the accurate mapping of NR functions in mouse liver, we characterized their levels of expression in whole liver as a function of time and diet, and explored NRs isoform expression in hepatocytes, cholangiocytes, Kupffer cells, hepatic stellate cells and liver sinusoidal cells. In addition, we leveraged liver single cell RNAseq studies to provide here an up-to-date compendium of NR expression in mouse liver in space and time.

ORGANISM(S): Mus musculus

PROVIDER: GSE223360 | GEO | 2023/05/24

REPOSITORIES: GEO

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Circadian transcriptomic analysis of mouse liver

Project description:Determination, using microarrays, of the mouse hepatic global programme of gene expression variations around the clock when animals had ad libitum food access The unique functional versatility of the liver is paramount for the organism homeostasis. Both liver development and adult life are controlled by tightly regulated transcription factor networks, among which nuclear receptors (NRs) regulate essential functions of parenchymal and nonparenchymal cells. Acting as transcription factors sensitive to extracellular cues such as steroidal hormones, lipid metabolites, xenobiotics… and modulated by intracellular signaling pathways, NRs orchestrate many aspects of hepatic physiology. While liver functional zonation and adaptability to fluctuating conditions are known to rely on a sophisticated cellular architecture, the exact functions of NRs in liver cell types remain poorly characterized. As a first step toward the accurate mapping of NR functions in mouse liver, we characterized their levels of expression in whole liver as a function of time and diet, and explored NRs isoform expression in hepatocytes, cholangiocytes, Kupffer cells, hepatic stellate cells and liver sinusoidal cells. In addition, we leveraged liver single cell RNAseq studies to provide here an up-to-date compendium of NR expression in mouse liver in space and time.

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