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PBMC immune transcriptome analysis in kids fed on doe's milk(Control) versus kids fed on replacer milk (Test) post-PPR vaccination

ABSTRACT: To study differential expression of genes in kids fed on doe's milk(Control) versus kids fed on replacer milk (Test) post PPR Vaccination

ORGANISM(S): Capra hircus

PROVIDER: GSE223506 | GEO | 2025/01/31

REPOSITORIES: GEO

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PBMC immune transcriptome analysis in kids fed on doe's milk(Control) versus kids fed on replacer milk (Test)

Project description:To study differential expression of genes in kids fed on does milk(Control) versus kids fed on replacer milk (Test)

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PBMC immune transcriptome analysis in kids fed on doe's milk(Control) versus kids fed on replacer milk (Test) post-PPR vaccination

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PBMC immune transcriptome analysis in kids fed on doe's milk(Control) versus kids fed on replacer milk (Test)

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MicroRNA profiling of mammary gland and liver tissue of cows under different feeding conditions (control, restricted amount of milk replacer, milk replacer ad libitum)

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Project description:Purpose: The present study was designed to identify both differentially expressed (DE) genes and differences in proteins accumulated in the liver tissues of suckling female lambs, thus trying to identify modified metabolic pathways as a consequence of milk restriction during the suckling period. Methods: Forty Assaf lambs (average BW 4.7 kg) were penned individually, twenty of them were fed milk replacer (200 g dry matter/L) ad libitum (ADL; 192 mL/kg LBW) whereas the other group (restricted, RES) only received 120 mL/kg LBW. When they were 35 days old, four animals per group were slaughtered (8 lambs in total) and a piece of liver was excised for transcriptomic analysis. The liver transcriptome analysis was carried out using RNA sequencing methodology (RNA-seq). Results: 386 DE genes were identified by RNA-seq, 198 of them being annotated genes in the KEGG pathway. Positive values of log2-fold change (log2FC) indicated that 210 genes were up-regulated in the liver of RES relative to the ADL group, whereas negative log2FC values denoted the down-regulation of 176 genes (P < 0.05). Conclusions: According to the data obtained, a restricted milk intake during the suckling period of replacement lambs affects hepatic transcriptome and proteome associated with an altered metabolism of lipids and proteins, thus reducing feed efficiency of replacement period.

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