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Targets of cytostatic drugs in HT1080 fibrosarcoma

ABSTRACT: HT1080 fibrosarcoma cells were treated with cytostatic drugs and analysed on Affymetrix microarray to identify genes and pathways regulated by these compounds. The array images were processed using Affymetrix MAS 5.0 software. and normalized by global scaling or scaled to a polyA+ spike mask Keywords = Cytostatic drugs Keywords = Actinomycin D Keywords = Doxorubicin Keywords = Vincristine Keywords = apoptosis Keywords: ordered

ORGANISM(S): Homo sapiens

PROVIDER: GSE2238 | GEO | 2006/02/04

SECONDARY ACCESSION(S): PRJNA91501

REPOSITORIES: GEO

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Transcription profiling of human HT1080 fibrosarcoma cells treated with cytostatic drugs

Project description:HT1080 fibrosarcoma cells were treated with cytostatic drugs and analysed on Affymetrix microarray to identify genes and pathways regulated by these compounds. The array images were processed using Affymetrix MAS 5.0 software. and normalized by global scaling or scaled to a polyA+ spike mask

2008-04-01 | E-GEOD-2238 | biostudies-arrayexpress

Expression data from RECK-overexpressing HT1080 fibrosarcoma cells

Project description:RECK, a glycosylphosphatidylinositol-anchored glycoprotein, inhibits the enzymatic activities of some matrix metalloproteinases (MMP), thereby suppressing tumor cell metastasis; however, the detailed mechanism is still obscure. In this study, we compared the gene expression profiles between mock- and RECK-transfected HT1080 cells. Three established cell lines were selected for RNA extraction and hybridization on Affymetrix microarrays: (1) mock-transfected HT1080 cells, designated as HT1080-Zeo, (2) RECK-overexpressing HT1080 cells, designated as HT1080-RECK, and (3) RECK/4NQ-overexpressing HT1080 cells (in which four N-glycosylation asparagine residues of RECK (Asn86, Asn200, Asn297, and Asn352) were replaced with glutamine residues), designated as HT1080-RECK/4NQ.

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Homo sapiens

Project description:Targets of cytostatic drugs in HT1080 fibrosarcoma

| PRJNA91501 | ENA

Expression data from RECK-overexpressing HT1080 fibrosarcoma cells

2010-04-14 | GSE21306 | GEO

Time-dependent expression data from human fibrosarcoma human cell line (HT1080) in collagen matrix with protease inhibitors

Project description:HT1080 cells were grown on collagen I and treated with PI for 4 h (short term) and 24 h (long term) while RNA was extracted for Genome-wide Affymetrix Microarray gene expression analysis. The objective of this study was to identify whether collagen accumulation driven by blocking proteases induced transcriptional changes that correlated sustained amoeboid cell state.

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Proteomic profiling of HT1080 fibrosarcoma cells of mesenchymal or amoeboid migratory phenotype

Project description:Amoeboid and mesenchymal migration of cancer cells both contribute to metastatic spreading of tumors. To characterize proteome changes underlying the different migratory modes, we performed mass spectrometry profiling of HT1080 fibrosarcoma cells undergoing mesenchymal-amoeboid transition induced by either doxycycline-inducible constitutively active RhoA or dasatinib treatment. Cells were kept in three-dimensional collagen gels with or without induction for 48 hours, lysed and processed for mass spectrometry analysis. In case of the inducible RhoA system, we also performed parallel two-dimensional experiments with cells on top of a dense layer of collagen (it was not feasible with dasatinib treatment).

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Effects of IFN beta treatment on gene expression in human fibrosarcoma HT1080 cells

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Suppression of MT1-MMP in Human Fibrosarcoma Impair Tumor Angiogenesis and Enlargement

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