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Effect of BFD2 (TGME49_311100) knockout on T. gondii gene expression during BFD1 induction

ABSTRACT: The acute-to-chronic stage transition in Toxoplasma gondii involes a global restructuring of the parasite transcriptome. Prior work identified a single transcription factor (BFD1) that is both necessary and sufficient to establish the chronic-stage program, making it the master regulator of this process. Recently, we found that parasites lacking a CCCH-type zinc finger RNA-binding protein (BFD2) are also unable to produce chronic forms. To understand how BFD2 aids in establishing the BFD1 transcriptional program, we knocked out BFD2 in a BFD1-conditional expression strain, wherein BFD1 is induced by treatment with the small molecule Shield-1.

ORGANISM(S): Toxoplasma gondii

PROVIDER: GSE223877 | GEO | 2023/03/31

REPOSITORIES: GEO

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