Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RREB1 Regulates Neuronal Proteostasis and Microtubule Network Function [ChIP-seq]

ABSTRACT: RREB1 regulates neuronal proteostasis and the microtubule network

ORGANISM(S): Mus musculus

PROVIDER: GSE223878 | GEO | 2024/01/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RREB1 Regulates Neuronal Proteostasis and Microtubule Network Function

Project description:RREB1 Regulates Neuronal Proteostasis and Microtubule Network Function

| PRJNA928716 | ENA

RREB1 Regulates Neuronal Proteostasis and Microtubule Network Function [RNA-seq]

Project description:RREB1 Regulates Neuronal Proteostasis and Microtubule Network Function [RNA-seq]

| PRJNA928721 | ENA

RREB1 Regulates Neuronal Proteostasis and Microtubule Network Function [ChIP-seq]

Project description:RREB1 Regulates Neuronal Proteostasis and Microtubule Network Function [ChIP-seq]

| PRJNA928722 | ENA

RREB1 Regulates Neuronal Proteostasis and Microtubule Network Function [RNA-seq]

Project description:To establish molecular pathways disrupted by loss of RREB1 in Purkinje cells, RNA-seq was performed on mRNA isolated from Purkinje cells and differential expression analysis was performed.

2024-01-01 | GSE223879 | GEO

RREB1 Regulates Neuronal Proteostasis and Microtubule Network Function

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-01-01 | GSE223881 | GEO

RNA-sequencing of Rreb1+/+ and Rreb1-/- embryonic day 7.5 mouse embryos

Project description:RNA-sequencing of Rreb1+/+ and Rreb1-/- embryonic day 7.5 mouse embryos

2021-04-21 | GSE148514 | GEO

RNA-sequencing of Rreb1+/+ and Rreb1-/- embryonic day 7.5 mouse embryos

Project description:RNA-sequencing of Rreb1+/+ and Rreb1-/- embryonic day 7.5 mouse embryos

| PRJNA624861 | ENA

Insulin Induces Microtubule Stabilization and Regulates the Microtubule Plus-End Tracking Protein Network in Adipocytes

Project description:Insulin-stimulated glucose uptake is known to involve microtubules, although the function of microtubules and the microtubule-regulating proteins involved in insulin action are poorly understood. CLASP2, a plus-end tracking microtubule-associated protein (+TIP) that controls microtubule dynamics, was recently implicated as the first +TIP associated with insulin-regulated glucose uptake. Here, using protein-specific targeted quantitative phosphoproteomics within 3T3-L1 adipocytes, we discovered that insulin regulates phosphorylation of the CLASP2 network members G2L1, MARK2, CLIP2, AGAP3 and CKAP5 as well as EB1, revealing the existence of a previously unknown microtubule-associated protein system that responds to insulin. To further investigate, G2L1 interactome studies within 3T3-L1 adipocytes revealed that G2L1 co-immunoprecipitates CLASP2 and CLIP2 as well as the master integrators of +TIP assembly, the end binding (EB) proteins. Live-cell total internal reflection fluorescence microscopy in adipocytes revealed G2L1 and CLASP2 colocalize on microtubule plus-ends. We found that while insulin increases the number of CLASP2-containing plus-ends, insulin treatment simultaneously decreases CLASP2-containing plus-end velocity. In addition, we discovered that insulin stimulates re-distribution of CLASP2 and G2L1 from exclusive plus-end tracking to “trailing” behind the growing tip of the microtubule. Insulin treatment increases -tubulin Lysine 40 acetylation, a mechanism that was observed to be regulated by a counterbalance between GSK3 and mTOR, and also led to microtubule stabilization. Our studies introduce insulin-stimulated microtubule stabilization and plus-end trailing of +TIPs as new modes of insulin action and reveal the likelihood that a network of microtubule-associated proteins synergize to coordinate insulin-regulated microtubule dynamics.

2019-06-13 | PXD011431 | Pride

Haploinsufficiency of RREB1 causes a Noonan-like syndrome via epigenetic reprogramming of RAS-MAPK pathway genes.

Project description:RREB1 is an epigenetic regulator of the RAS-MAPK pathway genes.

2020-03-13 | GSE146902 | GEO

Effect of RREB1 knockdown using siRNA in A673 Ewing sarcoma cells (RNA-seq)

Project description:Ewing sarcoma (EwS) is a rare bone and soft tissue malignancy driven by chromosomal translocations encoding chimeric transcription factors, such as EWSR1-FLI1, that bind GGAA motifs forming novel enhancers that alter nearby expression. We propose germline microsatellite variation at the 6p25.1 EwS susceptibility locus could impact downstream gene expression and EwS biology. We performed targeted long-read sequencing of EwS blood DNA to characterize variation and genomic features important for EWSR1-FLI1 binding. We identified 50 microsatellite alleles at 6p25.1 and observed EwS cases had longer alleles (>135 bp) with more GGAA repeats. The 6p25.1 GGAA microsatellite showed chromatin features of an EWSR1-FLI1 enhancer, and regulated expression of RREB1, a transcription factor associated with RAS/MAPK signaling. RREB1 knockdown reduced proliferation and clonogenic potential, and reduced expression of cell cycle and DNA replication genes. Our integrative analysis at 6p25.1 details increased binding of longer GGAA microsatellite alleles with acquired EWSR-FLI1 to promote Ewing sarcomagenesis by RREB1-mediated proliferation.

2023-10-23 | GSE220780 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data