Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Regulation of host RNA splicing in Mycobacterium tuberculosis infected macrophages

ABSTRACT: Here using a high-throughput yeast two-hybrid and mass spectrometry approach we show that mycobacterial proteins can access the RNA splicing machinery of the host, thereby playing a critical role in infection-induced perturbation of alternative splicing.

ORGANISM(S): Homo sapiens

PROVIDER: GSE223916 | GEO | 2025/03/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Host directed activity of Pyrazinamide in Mycobacterium tuberculosis infection

2013-06-28 | GSE48027 | GEO

Host directed activity of Pyrazinamide in Mycobacterium tuberculosis infection

Project description:Pyrazinamide (PZA) is one of the first line antibiotics used for the treatment of tuberculosis (TB). we have used human monocyte and a mouse model of pulmonary TB to investigate whether treatment with PZA, in addition to its known anti-mycobacterial properties, modulate the host immune response during Mycobacterium tuberculosis (Mtb) infection. Mice were infected with Mtb and treatment with PZA was started at 28 days post-infection. At 42 days and 63 days post-infection, group of animals were euthanized and lung tissue was collected to isolate total RNA and used in microarray experiments. Mtb-infected, untreated animals served as controls.

2013-06-28 | E-GEOD-48027 | biostudies-arrayexpress

Recognition of host-protective immune activation by Mycobacterium tuberculosis

Project description:The cytokine interferon-γ is a principal effector of macrophage activation and immune resistance to mycobacterial infection; however, pathogenic mycobacteria are capable of surviving in interferon-γ-activated macrophages by largely unknown mechanisms. We found that interferon-γ specifically bound to pathogenic mycobacteria and enhanced their growth in culture. Proteomic and electron microscopy analyses revealed that interferon-γ directly triggers proliferative activity and virulence phenotype in pathogenic mycobacteria that allow them to survive and grow inside macrophages. These findings suggest that pathogenic mycobacteria may have evolved eukaryotic-like signal transduction mechanisms to recognize host-protective immune activation.

2024-09-05 | PXD038542 | Pride

Mycobacterium tuberculosis

Project description:Characterization of mycobacterial oligopeptide permease system

| PRJNA118223 | ENA

A multi-omics investigation into the mechanisms of hyper-virulence in Mycobacterium tuberculosis

Project description:The result validated the connections between mutations, gene expression and mycobacterial pathogenicity, and identified new possible virulence-associated pathways in M. tuberculosis.

2022-07-13 | GSE203662 | GEO

Mycobacterium tuberculosis H37Ra

Project description:An attenuated strain used in mycobacterial virulence research

| PRJNA18649 | ENA

Identification of full length, splicing and cleavage products of SufB protein of SUF-complex of Mycobacterium tuberculosis

Project description:SufB, an Fe-S cluster assembly protein is critical for mycobacterial survival and virulence; but as a protein still at its nascent stage. SufB is interrupted by an intein sequence, which excises itself out from the full length protein post-translationally. Intein splicing is crucial for the activation of SufB, where intein(I) is excised and final functional product is generated by the ligation of flanking regions, known as Extein or Ligated extein(LE). Functional SufB is coordinating with other Suf proteins of SUF complex to generate Fe-S cluster. These are important for the central metabolism, gene expression, DNA repair etc. During the process of splicing, SufB has a tendency to generate off pathway products as N-cleavage (NC) and N-Extein (NE). In this project we have confirmed the identities of Mycobacterium tuberculosis full length SufB protein (precursor/p), SufB Intein (i), SufB Extein (ligated Extein/le), N-terminal cleavage product (nc) and N-extein (ne) by MALDI TOF/TOF mass spectrometry via gel based proteomics method .

2022-02-15 | PXD015199 | Pride

Mycobacterium tuberculosis

Project description:A novel phenotypic drug screen identifies potent inhibitors of mycobacterial virulence protein secretion

| PRJNA251986 | ENA

Host cholesterol modulates the generation and enrichment of persister population during Mycobacterium tuberculosis infection.

Project description:We previously reported that the utilization of host cholesterol is essential for mycobacterial persistence. In this study, we demonstrated that cholesterol-induced activation of a ribonuclease toxin (VapC12) inhibits translation by targeting proT tRNA in Mtb. The resulting cholesterol-specific growth modulation increases the frequency of the generation of persisters in a heterogeneous Mtb population. A vapC12-null mutant strain (ΔvapC12) failed to persist and demonstrated a hypervirulent phenotype in a guinea pig model of Mtb infection. This is the first study to identify a novel strategy through which cholesterol-specific activation of a toxin–antitoxin (TA) module in Mtb enhances persister formation during infection. In addition to identifying the mechanism, the study provides opportunity for targeting persisters, a new paradigm facilitating tuberculosis drug development.

2020-10-14 | PXD014323 | Pride

Global host transcriptional profiles following Mycobacterium avium subsp. paratuberculosis infection in mice

Project description:We focused on how Mycobacterium avium subsp. paratuberculosis influences the subsequent host response to investigate the host immunopathology accompanying the host anti-mycobacterial immune response during Mycobacterium avium subsp. paratuberculosis infection in spleen of mice.

2014-10-31 | GSE62836 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data