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Gene expression profile at single cell level of dendritic cells (DC) from the lung of neonatal (NEO) or adult (AD) mice

ABSTRACT: Multiple mechanisms restrain inflammation in neonates. Here we identify a population of pulmonary dendritic cells (DCs) that appear in the lungs and lung−draining lymph nodes between birth and two weeks of age and express intermediate levels of CD103 (CD103int). CD103int DCs express XCR1, CD205, and require the transcription factor, Batf3, suggesting that they belong to the cDC1 lineage. They also constitutively express CCR7 and spontaneously migrate to the lung−draining lymph node, where they promote stromal cell maturation and lymph node expansion, independently of microbial exposure and Trif− or MyD88−dependent signaling. CD103int DCs are transcriptionally related to efferocytic and tolerogenic DCs as well as mature, regulatory DCs. CD103int DCs efficiently acquire apoptotic cells and become activated through Mertk signaling, which also impairs CD8+ T cell activation. The appearance of CD103int DCs coincides with a temporal wave of apoptosis in developing lungs and explains, in part, impaired pulmonary immunity in neonatal mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE224178 | GEO | 2023/04/13

REPOSITORIES: GEO

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