Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

H2O2 Treatment of Human PBMC

ABSTRACT: To mimic the effect of ROS in contributing to ageing process in immune cells of PBMC. The PBMC cells were harvested from old human subjects (>50) and induced with H2O2 during 3 hour and 6 hour. The CEL file were normalized using RMA methods. We used microarray to explore the gene expression profiling before and after ROS. Gene related to cell cycle, apoptosis, metabolism and signal transduction were differentially expressed after inducing with H2O2 in compared to baseline.

ORGANISM(S): Homo sapiens

PROVIDER: GSE22463 | GEO | 2010/06/20

SECONDARY ACCESSION(S): PRJNA128627

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

H2O2 Treatment of Human PBMC

Project description:To mimic the effect of ROS in contributing to ageing process in immune cells of PBMC. The PBMC cells were harvested from old human subjects (>50) and induced with H2O2 during 3 hour and 6 hour. The CEL file were normalized using RMA methods. We used microarray to explore the gene expression profiling before and after ROS. Gene related to cell cycle, apoptosis, metabolism and signal transduction were differentially expressed after inducing with H2O2 in compared to baseline. Human PBMC cell were harvested before induce with H2O2 (T0), 3 hours (T3) and 6 hours (T6) from the older PBMC. Eight individual samples were collected and hybridized for each time point.

2010-07-08 | E-GEOD-22463 | biostudies-arrayexpress

Effecto of ROS (H2O2) on undifferentiated NSC

Project description:Effecto of ROS (H2O2) on undifferentiated NSC

| PRJNA975183 | ENA

Effect of ROS (H2O2) on undifferentiated NSC

Project description:Effect of ROS (H2O2) on undifferentiated NSC

| PRJNA971320 | ENA

Expression data provoked by H2O2 from plastid or peroxisomal origin

2014-09-30 | GSE54534 | GEO

NOD.scid, NOD, BDC2.5/NOD and BDC2.5/NOD.scid mice

Project description:Total pancreatic RNA was isolated from 3 week old NOD.scid, NOD, BDC2.5/NOD and BDC2.5/NOD.scid animals by GITC method. Targets were produced using standard Affymetrix procedures from about 10ug total RNA. The data from NOD.scid, NOD, BDC2.5/NOD and BDC2.5/NOD.scid Affymetrix MGU74Av2 cel files was converted into Robust Multi Array (RMA) text file for analysis using GeneSpring 6.1 Keywords: other

2005-01-26 | GSE1623 | GEO

Whole Transcriptome Sequencing (mRNA-seq) of cultured pulmonary primary endothelial cells treated with or without H2O2

Project description:ROS once released from myeloid cells is quickly converted into H2O2 in lungs. We performed RNAseq of cultured pulmonary primary endothelial cells treated with or without H2O2. Pathway enrichment analysis revealed that some of the signaling pathways that are altered by H2O2 are related to AKT signaling. AKT signaling has a protective role in a murine model of ALI by preventing capillary leakage. Moreover, H2O2 stimulates AKT activation in endothelial cells to strengthen vessel barrier integrity

2020-07-10 | GSE134373 | GEO

High-throughout sequencing of ovarian cancer cells treated with TMPyP4 and H2O2

Project description:The high-throughput sequencing technology was performed after the treatment of human ovarian cancer cells A2780 with TMPyP4 and H2O2 purchased from Sigma-Aldrich, to explore the expression of genes related to cell proliferation, DNA damage and ROS levels of ovarian cancer cells A2780 after the treatment of TMPyP4 and H2O2, and to find an interesting target pathway,HIF-1 signaling pathway.

2024-03-25 | GSE262015 | GEO

In vitro transcription studies used in a proof of concept whole transcriptome model predition study - A673 cells (1 of 4)

Project description:A673 cells were exposed in triplicate to three agrichemicals for 24hrs at 8 concentrations and a DMSO vehicle control (0.001, 0.003, 0.01, 0.03, 0.1, 0.3, 1, 3, and 10 μM plus DMSO vehicle controls). While a common set of DMSO controls was used, these CEL files were RMA normalized independently with each of the chemical treated groups. Gene expression was measured on an Affymetrix GeneTitan system. The compounds used were fenbuconazole (a.k.a FENB, CAS # 114369-43-6) a triazole fungicide, imazalil (a.k.a. IMAZ, CAS # 35554-44-0), an azole pesticide, and 2,4-dichlorophenoxyacetic acid (a.k.a. 2,4-D or 2-4-D in file names, CAS # 94-75-7), a chlorophenoxy herbicide. CEL files with _Rep2 in their file name indicate arrays that were re-scanned due to technical issues with the initial instrument scan of the GeneTitan peg array.

2018-12-21 | GSE109508 | GEO

Expression data provoked by H2O2 from plastid or peroxisomal origin

Project description:Reactive oxygen species (ROS) are key signalling molecules that regulate growth and development and coordinate responses to biotic and abiotic stresses. ROS homeostasis is controlled through a complex network of ROS production and scavenging enzymes. Recently, the first genes involved in ROS perception and signal transduction have been identified and, currently, we are facing the challenge to uncover the other players within the ROS regulatory gene network. The specificity of ensuing cellular responses depends on the type of ROS and their subcellular production sites. Various experimental systems, including catalase-deficient plants, in combination with genome-wide expression studies demonstrated that increased hydrogen peroxide (H2O2) levels significantly affect the transcriptome of plants and are capable of launching both defence responses and cell death events. We used microarrays to assess differential gene expression provoked by H2O2 from plastid or peroxisomal origin, respectively. Columbia-0 (Col-0, wild type), catalase-deficient Salk plants (10-15% of wild-type catalase activity; cat2-2; N576998; (Queval et al., 2007)) and A. thaliana plants expressing glycolate oxidase in chloroplasts (GO5 plants; (Fahnenstich et al., 2008)) were grown in soil under a 16h light/8h dark regime at photosynthetically active photon flux densities (PPFD) of 75 µmol quanta m-2 s-1 at 22°C day/18°C night temperatures and a CO2 concentration of 3,000 ppm. After three weeks of growth, plants were transferred to ambient CO2 concentration (380 ppm) and the same PPFD. Whole rosettes were harvested at 0h and 8h after transfer. Control samples were harvested at 8 h from plants continuously maintained in high CO2.

2014-09-30 | E-GEOD-54534 | biostudies-arrayexpress

Transcription profiling of mouse embryonic stem (ES) cells exposed to oxidative stress

Project description:Elevated reactive oxidative species (ROS) may be crucial for cellular transformation and tumor progression. Defining the response of stem cells to ROS is critical to understanding the unique sensitivity of stem cells to ROS-mediated transformation and tumor initiation. We examined both the immediate transcriptional and phospho-proteomic responses of murine ES cells induced by a single brief dose of hydrogen peroxide (H2O2) to create a more complete understanding of cell response to ROS. Experiment Overall Design: Murine ES cells were briefly exposed to low and high dose of H2O2 then allowed to recover for 1 hour in maintenance medium before RNA extraction and MG_U74Av2 microarray hybridization.

2009-11-10 | E-GEOD-18708 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data