Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing data of Mycobacterium marinum treated with compound 8

ABSTRACT: The mechanisms of action of compound 8 in Mycobacterium marinum although it inhibits the secretion of the bacteria. In this experiment, we aim to investigated the effect of compound 8 on the global gene expression of Mycobacterium marinum

ORGANISM(S): Mycobacterium marinum

PROVIDER: GSE224924 | GEO | 2023/02/14

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Characterization of the immune response to Mycobacterium marinum infection in zebrafish

Project description:Mycobacterium marinum infection in zebrafish (Danio rerio) has been widely used to study human tuberculosis because the bacteria causing these two diseases are close relatives. We studied the zebrafish immune response to M. marinum infection through a whole-genome level transcriptome analysis. As expected based on the literature, our results showed the induction of genes coding proteins associated to immune signaling, cell migration and acute phase response indicating that the immune response to M. marinum infection in zebrafish is similar than the response to tuberculosis causing Mycobacterium tuberculosis in humans.

2019-10-16 | GSE118288 | GEO

Novel compounds modulating Mycobacterium marinum ESX-5 secretion display anti-mycobacterial activity in vivo

Project description:We observed through Western blot that Mycobacterium marinum secreted more highly abundant PE-PGRS proteins that are above 130 kDa when treated with compound 36 than mock-treated bacteria. We would like to explore which PE-PGRS proteins are more secreted in this condition and whether other proteins are also differentially secreted between two conditions.

2023-01-19 | PXD037981 | Pride

miR-126 knockdown and Mycobacterium marinum infection

Project description:We identify a putative link between miR-126 knockdown and neuroactive ligand and notch signalling pathways in uninfected and Mycobacterium marinum infected zebrafish embryos.

2024-01-23 | GSE253537 | GEO

RNA sequencing data of Mycobacterium marinum treated with compound 8

Project description:RNA sequencing data of Mycobacterium marinum treated with compound 8

| PRJNA933127 | ENA

Zebrafish response to Mycobacterium marinum infection (Compugen series)

Project description:Mycobacteria infect macrophages that aggregate with additional macrophages and lymphocytes to form granulomas. We have used a functional genomics approach to identify immune response genes expressed during granuloma formation in Mycobacterium marinum-infected transparent zebrafish larvae where individual infection steps can be viewed in real time. We assessed RNA expression profiles from zebrafish larvae that were either infected with Mycobacterium marinum or mock-infected. Zebrafish infections were performed at 1 day post-fertilization (dpf), and samples were derived from pools of 6dpf zebrafish larvae. Keywords: host response to infection

2008-01-01 | GSE8846 | GEO

Zebrafish response to Mycobacterium marinum infection (Affymetrix series)

Project description:Mycobacteria infect macrophages that aggregate with additional macrophages and lymphocytes to form granulomas. We have used a functional genomics approach to identify immune response genes expressed during granuloma formation in Mycobacterium marinum-infected transparent zebrafish larvae where individual infection steps can be viewed in real time. We assessed RNA expression profiles from zebrafish larvae that were either infected with Mycobacterium marinum, mock-infected, or uninfected. Zebrafish infections were performed at 1 day post-fertilization (dpf), and samples were derived from pools of 6dpf zebrafish larvae. Keywords: host response to infection

2008-01-01 | GSE8327 | GEO

Comparative transcriptomics of Mycobacterium bovis and Mycobacterium marinum

Project description:Related surrogate species are often used to study the molecular basis of pathogenicity of a pathogen on the basis of a shared set of biological features generally attributable to a shared core genome consisting of orthologous genes. An important and understudied aspect, however, is the extent to which regulatory features affecting the expression of such shared genes are present in both species. Here we report on an analysis of whole transcriptome maps for an important member of the TB complex Mycobacterium bovis and a closely related model organism for studying mycobacterial pathogenicity Mycobacterium marinum. Predict transcription start site

2014-09-08 | E-GEOD-51881 | biostudies-arrayexpress

Characterization of the immune response to Mycobacterium marinum infection in zebrafish [mutant 463]

Project description:Mycobacterium marinum infection in zebrafish (Danio rerio) has been widely used to study human tuberculosis because the bacteria causing these two diseases are close relatives. We studied the zebrafish immune response to M. marinum infection through a whole-genome level transcriptome analysis. In addition, we carried out a medium-scale forward genetic screen to identify genes underlying defense mechanisms against M. marinum infection in zebrafish. Our aim was to gain more information about the genetic mechanisms important in the immune defense against human tuberculosis. In this screen, we identified a mutant zebrafish line with impaired resistance to a low-dose M. marinum infection. In this line, the transcriptome analysis at 14 days post infection revealed decreased expression of a gene homologous to human UNC119 which has been shown to have a role in T cell activation.

2019-10-16 | GSE118350 | GEO

Accelerating early anti-TB drug discovery by creating mycobacterial indicator strains that predict mode of action [Mycobacterium marinum]

Project description:Due to the rise of drug resistant forms of tuberculosis there is an urgent need for novel antibiotics to effectively combat these cases and to shorten treatment regimens. Recently, drug screens using whole cell analyses have shown to be successful. However, current high throughput screens focus mostly on stricto sensu life-death screening that give little qualitative information and often require the lengthy process of target and mode of action (MoA) identification. In doing so, promising compound scaffolds or non-optimized compounds that fail to reach inhibitory concentrations are missed. To accelerate early TB drug discovery, we performed RNA sequencing on Mycobacterium tuberculosis and Mycobacterium marinum to map the stress responses that follow upon exposure to sub-inhibitory concentrations of antibiotics with known targets: ciprofloxacin, ethambutol, isoniazid, streptomycin and rifampicin. The resulting dataset comprises the first overview of transcriptional stress responses of mycobacteria to different antibiotics. We show that antibiotics can be distinguished based on their specific transcriptional stress fingerprint i.e. DNA damage for ciprofloxacin and ribosomal stress for streptomycin. Notably, this fingerprint was more distinctive in M. marinum and we decided to use this to our advantage and continue with this model organism. A selection of diverse antibiotic stress genes was used to construct stress reporters. In total, three functional reporters were constructed for DNA damage, cell wall damage and ribosomal inhibition. Subsequently, these reporter strains were used to screen a small anti-TB compound library to predict the mode of action. In doing so we could identify the putative mode of action for three novel compounds, which confirms our approach.

2018-04-12 | GSE107882 | GEO

Comparative transcriptomics of Mycobacterium bovis and Mycobacterium marinum

2014-09-08 | GSE51881 | GEO