Autophagy-overactivated composite microbe engineered from oncolytic adenoviruses for the cascade enhancement of cancer immunotherapy
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ABSTRACT: Cell derived xenografts treated with OMVs@P2O-Ads or PBS were collected and then the total RNA was isolated. After removal of the remaining genomic DNA, mRNA was purified from total RNA using polyT and then fragmented with 10× RNA fragmentation buffer and the RNA-seq library was constructed using Hieff NGS Ultima Dual-mode mRNA Library Prep Kit and was sequenced.
Project description:RAW264.7 cells treated with GB2 or not were collected and then the total RNA was isolated. After removal of the remaining genomic DNA, MRNA was purified from total RNA using polyT and then fragmented with 10x RNA fragmentation buffer and the RNA-seg library was constructed using Hieff NGS Ultima Dual-mode mRNA Library Prep Kit and was sequenced.
Project description:B16F10 cells treated with DMSO or BRQ were collected and then the total RNA was isolated. After removal of the remaining genomic DNA, mRNA was purified from total RNA using polyT and then fragmented with 10× RNA fragmentation buffer and the RNA-seq library was constructed using Hieff NGS Ultima Dual-mode mRNA Library Prep Kit and was sequenced.
Project description:MC38 tumors from C57BL/6 mice treated with GB2 or PBS were collected and then the total RNA was isolated. After removal of the remaining genomic DNA, MRNA was purified from total RNA using polyT and then fragmented with 10x RNA fragmentation buffer and the RNA-seg library was constructed using Hieff NGS Ultima Dual-mode mRNA Library Prep Kit and was sequenced.
Project description:Cell derived xenografts treated with DMSO, Oxa, APAP or OAP2 were collected and then the total RNA was isolated. After removal of the remaining genomic DNA, mRNA was purified from total RNA using polyT and then fragmented with 10× RNA fragmentation buffer and the RNA-seq library was constructed using Hieff NGS Ultima Dual-mode mRNA Library Prep Kit and was sequenced.
Project description:~1500 K562 cells expressing dCas9-KRAB were profiled on the 10x Genomics 3' v3 droplet-based scRNA-seq platform. The resulting library was sequenced on the Illumina and Ultima sequencing platforms.
Project description:Characterization of the sRNA content of P. aeruginosa OMVs compared to whole cells. Result: OMVs contain differentially packaged sRNAs. Whole cell PA14 and OMVs from 3 separate preparations.
Project description:This study compares the gene expression level in four cancer cell lines before and after vinigrol treatment. The Lexogen QuantSeq 3'mRNA-seq library kit or Hieff NGS MaxUp II Dual-mode mRNA Library Prep Kit for Illumina MaxUp II was used to prepare the RNA-seq libraries in this study. We identified the dysregulated genes during this progress and explored the potential mechanism of vinigrol induced cell death in breast cancer cells. Overall design: MCF7-ADR, ,MDA-MB-231 and T47D cells treated with vinigrol (50 micromoles) for 6 hours compared with the DMSO group.