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A soybean pattern recognition receptor conferring broad-spectrum pathogen and pest resistance regulates expression of several NLR receptor proteins

ABSTRACT: Understanding the molecular response of plants to innumerable pathogens and pests is crucial in managing and eradicating plant diseases. We investigated the transcriptomic depiction of GmDR1 transgenic lines vs. their genitor Williams 82 treated with the PAMP chitin to critical gene expression changes that might lead to the defense responses manifested by the GmDR1 transgenic plants

ORGANISM(S): Glycine max

PROVIDER: GSE226254 | GEO | 2023/02/28

REPOSITORIES: GEO

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The Northern Corn Leaf Blight Resistance Gene, Ht1, Encodes an NLR Immune Receptor

Project description:Northern corn leaf blight (NLB), caused by the fungal pathogen Exserohilum turcicum, results significant yield reductions in infected corn. The first major locus conferring resistance to E. turcicum race 0, Ht1, was identified over 50 years ago, but despite widespread deployment the underlying gene has remained unknown. We employed map-based cloning to identify the Ht1 causal gene, which was found to be a coiled-coil nucleotide-binding, leucine-rich repeat (NLR) gene, termed PH4GP-Ht1. Transgenic testing confirmed that addition of the native PH4GP-Ht1 sequence to the susceptible maize variety PH184C resulted in resistance to E. turcicum race 0. A survey of the maize NAM genomes revealed that susceptible Ht1 alleles had very low to no expression, but overexpression of the susceptible B73 allele did not result in resistant plants, indicating that relatively minor protein sequence variations may underlie the resistance phenotype. Modeling of the PH4GP-Ht1 protein indicated that it has structural homology to the Arabidopsis NLR resistance gene ZAR1, and likely forms a similar homo-pentamer structure following activation. RNA-seq data from an infection time course revealed that one week after inoculation there was a threefold reduction in fungal biomass and a dramatic increase in DEGs when comparing mock to inoculated PH4GP-Ht1 transgenic plants and null plants. These results demonstrate that the NLR PH4GP-Ht1 is the causal gene underlying the NLB resistance phenotype of Ht1.

2022-10-03 | GSE206951 | GEO

A soybean pattern recognition receptor conferring broad-spectrum pathogen and pest resistance regulates expression of several NLR receptor proteins

Project description:A soybean pattern recognition receptor conferring broad-spectrum pathogen and pest resistance regulates expression of several NLR receptor proteins

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Identification of the Capsicum baccatum NLR Protein CbAR9 Conferring Disease Resistance to Anthracnose.

Project description:Anthracnose is caused by Colletotrichum species and is one of the most virulent fungal diseases affecting chili pepper (Capsicum) yield globally. However, the noble genes conferring resistance to Colletotrichum species remain largely elusive. In this study, we identified CbAR9 as the causal locus underlying the large effect quantitative trait locus CcR9 from the anthracnose-resistant chili pepper variety PBC80. CbAR9 encodes a nucleotide-binding and leucine-rich repeat (NLR) protein related to defense-associated NLRs in several other plant species. CbAR9 transcript levels were induced dramatically after Colletotrichum capsici infection. To explore the biological function, we generated transgenic Nicotiana benthamiana lines overexpressing CbAR9, which showed enhanced resistance to C. capsici relative to wild-type plants. Transcript levels of pathogenesis-related (PR) genes increased markedly in CbAR9-overexpressing N. benthamiana plants. Moreover, resistance to anthracnose and transcript levels of PR1 and PR2 were markedly reduced in CbAR9-silenced chili pepper fruits after C. capsici infection. Our results revealed that CbAR9 contributes to innate immunity against C. capsici.

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GmMPK4 is a negative regulator of SA-mediated defense response

Project description:GmMPK4 silencing was performed in Glycine max (Williams 82) using virus-induced gene silencing (VIGS) mediated by Bean pod mottle virus (BPMV). Microarray analysis was performed to compare the transcriptional profile of GmMPK4-silenced plants vs vector control plants. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Jianzhong Liu. The equivalent experiment is GM25 at PLEXdb.] genotype: Williams 82(4-replications) genotype: GmMPK4-silenced Williams 82(4-replications)

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Role of MPK4 in pathogen-associated molecular pattern-triggered alternative splicing in Arabidopsis

Project description:Alternative splicing (AS) of pre-mRNAs in plants is an important mechanism of gene regulation in environmental stress tolerance but plant signals involved are essentially unknown. Pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) is mediated by mitogen-activated protein kinases and the majority of PTI defense genes are regulated by MPK3, MPK4 and MPK6. These responses have been mainly analyzed at the transcriptional level, however many splicing factors are direct targets of MAPKs. Here, we studied alternative splicing induced by the PAMP flagellin in Arabidopsis.

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A conserved module regulates receptor kinase signaling in immunity and development

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Comparative expression analysis of roots of two soybean cultivars with contrasting drought-tolerant phenotype under well-watered and dehydration conditions.

Project description:To understand the responses of plants to environmental stresses will help mitigate the problems via creating stress-tolerant crop cultivars. We have carried out comparative expression analysis of roots of two soybean varieties Williams 82 and DT2008 that have constrasting drought-responsive phenotype under dehydration and well-watered (control) conditions. Affymetrix’s whole Soybean Gene Expression Microarray (66K) was used. The Williams 82 and DT2008 soybean plants were grown for 14 days in the vermiculite soil under greenhouse conditions. The whole plants of 14-d-old plants were detached and exposed to dehydration on KimTowel papers for 0 (well-watered, control), 2 and 10 h. All roots of independent 14-d-old plants were collected. Total RNA was prepared and used for the microarray hybridization. Three independent biological replicates were used for each plant sample.

2015-07-02 | E-GEOD-65553 | biostudies-arrayexpress

GmMPK4 is a negative regulator of SA-mediated defense response

2011-06-02 | GSE29653 | GEO

Transcript profiling upon ethanol-induced expression of Xcv type 3- effector xopB in A. thaliana

Project description:The bacterial pathogen Xanthomonas campestris pv. vesicatoria 85-10 (Xcv) translocates about 30 type 3 effector proteins (T3Es) into pepper plants (Capsicum annuum) to suppress its immune responses. Among them is XopB which was previously shown to interfere with cell wall-bound invertase (CwInv) and sugar-mediated defence responses. Transgenic Arabidopsis thaliana lines expressing xopB under control of an ethanol inducible promotor were generated to investigate the in planta activity of this effector protein in more depth. The microarray analysis revealed that expression of the bacterial T3E XopB in A. thaliana plants causes immediate and massive reprogramming of transcription resulting in the alteration of the hormonal homeostasis and imbalances in signalling cascades. Further analyses unravelled that XopB modifies the pathogen associated molecular pattern (PAMP)-triggered calcium signature and inhibits the burst of reactive oxygen species (ROS).

2015-10-01 | GSE66477 | GEO

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