Assessing kidney injury induced by mercuric chloride in guinea pigs with in vivo and in vitro experiments [in vivo]
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ABSTRACT: In this study we tested the ability to predict organ injury from in vivo transcriptomics data in male Hartley guinea pigs at early time points after exposure to mercury chloride (9 and 33 hours). We selected mercury chloride, a compound extensively used in animal studies for its ability to cause acute kidney damage.
Project description:In this study we tested the ability to predict organ injury from in vitro transcriptomics data at early time points after exposure to mercury chloride (12 and 24 hours). We selected mercury chloride, a compound extensively used in animal studies for its ability to cause acute kidney damage.
Project description:In this study we tested the ability to predict organ injury from transcriptomics data in Sprague-Dawley rats at early time points after exposure to mercury chloride (10 and 34 hours). We selected mercury chloride, a compound extensively used in animal studies for its ability to cause acute kidney and liver damage.
Project description:Non-alcoholic steatohepatitis (NASH) is a serious health challenge affecting millions worldwide, and research advances are restricted by the limited availability of preclinical models recapitulating the complex disease etiology and hepatic histopathology. Uniquely, the diet induced guinea pig model develops NASH with fibrosis resembling human histopathology however, no data is available depicting the guinea pig NASH transcriptome. We provide the first high throughput sequencing results on guinea pig NASH with advanced fibrosis. Transcriptomic profiles in guinea pig NASH clearly separated from controls, and pathways involved in fibrosis, inflammation and lipid metabolism were all highly regulated.
Project description:In this study we tested the ability to predict organ injury from transcriptomic data in Hartley guinea pigs at early time points after exposure to thioacetamide (9 and 33 hours). We selected thioacetamide, a compound extensively used in animal studies for its ability to cause liver damage.