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Analysis of gene expression in different T cell clones

ABSTRACT: The aim was to analyze the expression of genes in Th17 cell clones derived from a patient affected by multiple sclerosis before and after activation with anti-CD3 and anti-CD28. Keywords: Gene expression, T cell clones, Th17 characterisation

ORGANISM(S): Homo sapiens

PROVIDER: GSE22642 | GEO | 2010/07/01

SECONDARY ACCESSION(S): PRJNA128039

REPOSITORIES: GEO

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Analysis of gene expression in different T cell clones

Project description:The aim was to analyze the expression of genes in Th17 cell clones derived from a patient affected by multiple sclerosis before and after activation with anti-CD3 and anti-CD28. Keywords: Gene expression, T cell clones, Th17 characterisation The T cell clones were derived according to well known protocol, using different cytokines, and classified on the basis of their ability to produce cytokines. Once characterized for their production of different cytokines, they were stimulated with anti-CD23 and anti-CD28 and used to prepare microarray experiments comparing the stimulated clones against the unstimulated ones.

2010-07-01 | E-GEOD-22642 | biostudies-arrayexpress

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Analysis of gene expression in different T cell clones

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Analysis of gene expression in different T cell clones

Project description:The aim was to analyze the expression of genes in T cell clones having different functional profiles (Th1, Th0, Th17, Th2) obtained from the same individual. Keywords: Gene expression, T cell clones, Th17 characterisation The T cell clones were derived according to well known protocol, using different cytokines, and classified on the basis of their ability to produce cytokines. Once characterized for their production of IL-4, IL-12, gamma-IFN, IL-13, IL-1, they were used to prepare microarray experiments.

2008-05-30 | E-GEOD-11553 | biostudies-arrayexpress

Transcription profiling of human Treg and Th clones - experiment I

Project description:The aim of this work was to identify functional features that are specific of human Treg cells, through the identification of genes that are differentially expressed: 1/ in activated Treg clones versus activated Thelper clones; 2/ in Th clones activated in the presence versus the absence of TGFb; 3/ in suppressed Th clones, i.e. Th clones activated in the presence of Treg clones, versus controls. Experiment Overall Design: Two to four million cells of human T cell clones were collected at rest (time point = 0) or after activation with coated anti-CD3 and soluble anti-CD28 antibodies (time points = 6, 24 or 41hrs). RNA was extracted for hybridization on Affymetrix microarrays.

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Expression data measured by custom Nanostring gene set of CD4+ T cells from healthy individuals stimulated with anti-CD3/CD28 with or without IFNb or Th17 polarizing cytokines

Project description:Variation in individuals' adaptive immune response is believed to influence susceptibility to complex diseases in humans. The genetic basis of such variation is poorly understood. We measured gene expression from resting and activated CD4+ T cells derived from the peripheral blood of 348 healthy individuals. We activated the primary T cells with anti-CD3/CD28 beads. We collected peripheral blood from each human donor. We isolated peripheral blood mononuclear cells by Ficoll, and negatively selected for CD4+ T cells using RosettaSep. We isolated peripheral blood mononuclear cells by Ficoll, and negatively selected for CD4+ T cells using RosettaSep. We then either left cells unstimulated or stimulated them with beads conjugated with anti-CD3 and anti-CD28 either without additional cytokines, or with IFNb, or with Th17 cocktail. Cells were harvest at 0hr, 4hr (anti-CD3/CD28 +/- IFNb) or 48hr (anti-CD3/CD28 +/- Th17), lysed and RNA isolated to be profiled on Nanostring.

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IL-1β induces the rapid secretion of the antimicrobial protein IL-26 from Th17 cells

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2017-11-10 | E-MTAB-5739 | biostudies-arrayexpress

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Project description:Variation in individuals' adaptive immune response is believed to influence susceptibility to complex diseases in humans. The genetic basis of such variation is poorly understood. We measured gene expression from resting and activated CD4+ T cells derived from the peripheral blood of healthy individuals. We activated the primary T cells with anti-CD3/CD28 beads alone or with IFNb or Th17 polarizing cytokines. We collected peripheral blood from each human donor. We isolated peripheral blood mononuclear cells by Ficoll, and negatively selected for CD4+ T cells using RosettaSep. We then either left cells unstimulated or stimulated them with beads conjugated with anti-CD3 and anti-CD28 either without additional cytokines, or with IFNb, or with Th17 cocktail. Cells were harvest at up to 8 time points (0hr, 45min, 2hr, 4hr, 10hr, 24hr, 48hr and 72hr), lysed and RNA isolated to be profiled on microarray.

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