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Neurospora crassa early sexual development with MTP priming

ABSTRACT: Multi-targeting priming (MTP) for genome-wide gene expression assays provides selective targeting of multiple sequences and counter-selection against undesirable sequences. We demonstrated superior performance of two MTPs compared to oligo-dT microarray profling and RNA tag sequencing the response of Saccharomyces cerevisiae to nitrogen deficiency and profiling Neurospora crassa early sexual development. Priming with MTPs in addition to oligo-dT resulted in higher sensitivity, a greater number of well-measured genes, more genes significantly differentially expressed, and a greater power to detect meager differences.

ORGANISM(S): Neurospora crassa

PROVIDER: GSE22658 | GEO | 2010/07/03

SECONDARY ACCESSION(S): PRJNA133081

REPOSITORIES: GEO

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