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Saccharomyces cerevisiae grown in nitrogen depletion with MTP priming

ABSTRACT: Multi-targeting priming (MTP) for genome-wide gene expression assays provides selective targeting of multiple sequences and counter-selection against undesirable sequences. We demonstrated superior performance of two MTPs compared to oligo-dT microarray profling and RNA tag sequencing the response of Saccharomyces cerevisiae to nitrogen deficiency and profiling Neurospora crassa early sexual development. Priming with MTPs in addition to oligo-dT resulted in higher sensitivity, a greater number of well-measured genes, more genes significantly differentially expressed, and a greater power to detect meager differences. Saccharomyces cerevisiae S288c was grown on two different media.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Francesc Lopez

PROVIDER: E-GEOD-22936 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Multi-targeted priming for genome-wide gene expression assays.

Adomas Aleksandra B AB Lopez-Giraldez Francesc F Clark Travis A TA Wang Zheng Z Townsend Jeffrey P JP

BMC genomics 20100817

<h4>Background</h4>Complementary approaches to assaying global gene expression are needed to assess gene expression in regions that are poorly assayed by current methodologies. A key component of nearly all gene expression assays is the reverse transcription of transcribed sequences that has traditionally been performed by priming the poly-A tails on many of the transcribed genes in eukaryotes with oligo-dT, or by priming RNA indiscriminately with random hexamers. We designed an algorithm to fin ...[more]

PMID: 20716356

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Project description:Multi-targeting priming (MTP) for genome-wide gene expression assays provides selective targeting of multiple sequences and counter-selection against undesirable sequences. We demonstrated superior performance of two MTPs compared to oligo-dT microarray profling and RNA tag sequencing the response of Saccharomyces cerevisiae to nitrogen deficiency and profiling Neurospora crassa early sexual development. Priming with MTPs in addition to oligo-dT resulted in higher sensitivity, a greater number of well-measured genes, more genes significantly differentially expressed, and a greater power to detect meager differences. Saccharomyces cerevisiae S288c was grown on two different media and two different primers were used for reverse transcription: oligo(dT) and Multi-Targeted Primer [MTP] protoperithecia oligo(dT)+ MTP (PP2) perithecia oligo(dT)+ MTP (PT2). Two biological replicates and dye swapping

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Saccharomyces cerevisiae

Project description:Saccharomyces cerevisiae grown in nitrogen depletion with MTP priming

| PRJNA133099 | ENA

Saccharomyces cerevisiae mRBPome

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