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Della-regulation in darkness versus light-DELLAs contribute to skotomorphogenesis

ABSTRACT: rs09-09_della-dark - della-regulation in darkness versus light - Identification of DELLA-dependent downstream targets in darkness - Aim was to determine downstream target of DELLA proteins involved in skotomorphogenesis. Wt, ga1-3, ga1-3_rga_gai_rgl1_rgl2_rgl3 global seeds were sterilized, sown on MS agar plates then put for stratification for 3 days at 4°C. Plates were placed in growth cabinet at 22°C for 5 days in darkness or in continous light. Keywords: gene knock out

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE22681 | GEO | 2011/06/13

SECONDARY ACCESSION(S): PRJNA128333

REPOSITORIES: GEO

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della-regulation in darkness versus light-DELLAs contribute to skotomorphogenesis

Project description:rs09-09_della-dark - della-regulation in darkness versus light - Identification of DELLA-dependent downstream targets in darkness - Aim was to determine downstream target of DELLA proteins involved in skotomorphogenesis. Wt, ga1-3, ga1-3_rga_gai_rgl1_rgl2_rgl3 global seeds were sterilized, sown on MS agar plates then put for stratification for 3 days at 4Â°C. Plates were placed in growth cabinet at 22Â°C for 5 days in darkness or in continous light. Keywords: gene knock out 9 dye-swap - CATMA arrays

2011-06-12 | E-GEOD-22681 | biostudies-arrayexpress

della-regulation of salt stress responses-DELLAs contribute to salt stress responses

Project description:rs06-07_della - della-regulation of salt stress responses - Identification of DELLA-dependent dowtream targets in response to salt stress - Aim was to determine downstream target of DELLA proteins involved in salt stress tolerance. Wt, ga1-3, penta seeds were sterilized, sown on MS agar plates then put for stratification for 3 days at 4degreeC. Plates were placed in growth cabinet for 9 days. Seedlings were transferred to 24 well-plates, with 2 seedlings per well (0.5 ml MS liquid per well). Plates were placed in the same condition for 3 days. Finally, NaCl were added (final concentration 200 mM), except for the control. The salt treatment was applied for 30 min and 1h. Treatment was stopped by freezing in liquid nitrogen. Keywords: dose response,gene knock out,time course

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della-regulation of salt stress responses-DELLAs contribute to salt stress responses

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Identification of target genes of the DELLA protein RGL2 during the repression of seed germination

Project description:To shed light on the genetic events downstream of DELLA proteins, we have employed a microarray expression profiling of Arabidopsis thaliana seeds to identify target genes of the DELLA protein RGL2. Seeds of the germinating ga1-3 rga-t2 rgl2-1 and the non-germinating ga1-3 rga-t2 mutant were stratified, and RNA was extracted after five days. Transcript profiles of the non-germinating seeds closely resemble profiles of dormant seeds, and several transcription factors involved in light- and phytohormone-regulated signalling pathways appear to be up-regulated, suggesting that RGL2 controls various physiological aspects to inhibit seed germination.

2012-08-31 | GSE40485 | GEO

Identification of target genes of the DELLA protein RGL2 during the repression of seed germination

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comparison of transcriptomes in mad mutants-Transcriptome analysis of miRNA action deficient mutants

Project description:rs10-04_mad - comparison of transcriptomes in mad mutants - What gene sets are differentially expressed in mad mutants? - Seeds of GFP171.1 (parental line), mad1, mad2, mad3, mad6 and dcl1-12 were sterilized and germinated on Murashige/Skoog medium with 0.9% agar. Plates were stratified at 4C in the dark for 4 days. The plates were then transferred to a growth cabinet at 21C under a 16h light/8h darkness light regime, and the seedlings were harvested 18 days after transfer to the growth cabinet.

2011-09-15 | GSE30477 | GEO

Arabidopsis thaliana

Project description:della-regulation in darkness versus light-DELLAs contribute to skotomorphogenesis

| PRJNA128333 | ENA

Identification of early target genes of the DELLA protein GAI in etiolated seedlings

Project description:The hormones gibberellins (GA) control many aspects of plant growth and development thruough the whole life cycle of the plant. For instance, GA participate in the establishment of the skotomorphogenic developmental program that is triggered when seeds germinate in darkness, i.e. under the soil. Under these conditions seedlings appear etiolated and developmental features usually triggered by light are kept repressed. The GA signaling elements GAI and RGA have a major, partially redundant role in this process. These proteins belong to the DELLA family of transcriptional regulators and are destabilized by GA, acting as negative regulators of the pathway. In order to understand the molecular basis of the regulation of the skotomorphogenesis by GA, we sought to identify early target genes of the activity of GAI in etiolated seedlings. For that purpose we analyzed rapid, global changes in gene expression in response to a transient accummulation of a dominant version of GAI, gai-1, which is resistant to GA-induced destabilization.

2011-09-01 | GSE24253 | GEO

Phosphorylation Promotes DELLA Activity by Enhancing Its Binding to Histone H2A at Target Chromatin in Arabidopsis

Project description:DELLA proteins are conserved master growth regulators that play a central role in controlling plant development in response to internal and environmental cues. DELLAs function as transcription regulators, which are recruited to target promoters by binding to transcription factors (TFs) and histone H2A via its GRAS domain. Recent studies showed that DELLA stability is regulated post-translationally via two mechanisms, phytohormone gibberellin-induced polyubiquitination for its rapid degradation, and Small Ubiquitin-like Modifier (SUMO)-conjugation to alter its accumulation. Moreover, DELLA activity is dynamically modulated by two distinct glycosylations: DELLA-TF interactions are enhanced by O-fucosylation, but inhibited by O-linked N-acetylglucosamine (O-GlcNAc) modification. However, the role of DELLA phosphorylation remains unclear. Here, we identified phosphorylation sites in REPRESSOR OF ga1-3 (RGA, an AtDELLA) purified from Arabidopsis by tandem mass spectrometry analysis, and showed that phosphorylation of the RGA LKS-peptide in the poly-S/T region enhances RGA-H2A interaction and RGA association with target promoters. Interestingly, phosphorylation does not affect RGA-TF interactions. Our study has uncovered that phosphorylation is a new regulatory mechanism of DELLA activity.

2024-08-08 | PXD046004 | Pride

comparison of transcriptomes in mad mutants-Transcriptome analysis of miRNA action deficient mutants

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