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Effect of the marine natural product-derived compound ZJ-101 on MDA-MB-231 gene expression in 2D culture format

ABSTRACT: Four logs of ZJ-101 were tested on 2D cultures of MDA-MB-231 cells to identify relevant mechanistic pathways through differentially expressed gene analysis

ORGANISM(S): Homo sapiens

PROVIDER: GSE227790 | GEO | 2023/05/31

REPOSITORIES: GEO

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Effect of the marine natural product-derived compound ZJ-101 on gene expression in MDA-MB-231 3D Spheroids

Project description:Four logs of ZJ-101 were tested on 3D spheroid cultures of MDA-MB-231 cells to identify relevant mechanistic pathways through differentially expressed gene analysis

2023-05-31 | GSE227989 | GEO

Effect of the marine natural product-derived compound ZJ-101 on MDA-MB-231 cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-05-31 | GSE231359 | GEO

Effect of the marine natural product-derived compound ZJ-101 on MDA-MB-231 gene expression in 2D culture format

Project description:Effect of the marine natural product-derived compound ZJ-101 on MDA-MB-231 gene expression in 2D culture format

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Effect of the marine natural product-derived compound ZJ-101 on MDA-MB-231 cells

Project description:Effect of the marine natural product-derived compound ZJ-101 on MDA-MB-231 cells

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Effect of the marine natural product-derived compound ZJ-101 on gene expression in MDA-MB-231 3D Spheroids

Project description:Effect of the marine natural product-derived compound ZJ-101 on gene expression in MDA-MB-231 3D Spheroids

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The Marine Natural Product Furospinulosin 1 Induces Apoptosis in MDA-MB-231 Triple Negative Breast Cancer Cell Spheroids, But Not in Cells Grown Traditionally with Longer Treatment.

Project description:Cancer cells grown in spheroid conditions interact with each other and the extracellular matrix, providing a better representation of the in vivo environment than two-dimensional cultures and are a more clinically relevant model. A discrete screening of genetically diverse marine samples in the spheroid assay led to the identification of a novel activity for the known compound furospinulosin 1. This compound shows activity against MDA-MB-231 triple negative breast cancer cells grown as spheroids and treated for 24 or 48 h. No cytotoxicity was seen in traditional two-dimensional adherent cultures treated for a longer time (72 h). A reverse phase protein array (RPPA) confirmed the limited activity of the compound in cells grown traditionally and revealed changes in protein expression when cells are grown as spheroids that are associated with better clinical prognosis. Analysis of the RPPA data through the Broad institute's connectivity map suggested the hypothesis that furospinulosin 1 functions as an MEK inhibitor. Analysis of the RPPA data through STRING supports the apoptosis observed. The selectivity exhibited by furospinulosin 1 for triple negative breast cancer cells only when grown as spheroids makes it an interesting compound with strong therapeutic potential that merits further study.

| S-EPMC8145321 | biostudies-literature

RNA-seq BHT-101 cells and BHT-101 ASH1L KO cell lines

Project description:The goal of this study is to assess the role of ASH1 like histone lysine methyltransferase (ASH1L) in the biology of anaplastic thyroid cancer. CRISPR-Cas9 was used to create 4 independent cell lines derived from BHT-101 anaplastic thyroid cancer cells with premature stop codons prior to the catalytic domain within both alleles of ASH1L. RNA-seq was performed on these 4 KO cell lines, and compared to 3 biological replicates of wild type BHT-101 cells.

2020-05-09 | GSE147076 | GEO

ChIP-seq BHT-101 cells and BHT-101 ASH1L KO cell lines

Project description:The goal of this study is to assess the role of ASH1 like histone lysine methyltransferase (ASH1L) in the biology of anaplastic thyroid cancer. CRISPR-Cas9 was used to create cell lines derived from BHT-101 anaplastic thyroid cancer cells with premature stop codons prior to the catalytic domain within both alleles of ASH1L. ChIP-seq for H3K36me2, the histone mark catalyzed by ASH1L, was performed on two KO cell lines, and compared to wild type BHT-101 cells.

2020-05-09 | GSE147074 | GEO

microRNA-101 represses EZH2 in cancer

Project description:To determine the phenotypic overlap between restoring miR-101 expression in bladder cancer cells and knocking down EZH2. Either control precursors, pre-miR-101, control siRNA, or EZH2 siRNA were transfected in triplicate into UM-UC-3 cells for 72 hours at a final concentration of 50 nM.

2010-06-25 | E-GEOD-13674 | biostudies-arrayexpress

Regulation of gene expression by miR-101 in U2OS cells

Project description:miR-101 is a tumor suppressive microRNA. Besides EZH2, a well known EZH2 target, miR-101 should have other targets, which might also contribute to its tumor suppressive function. Thus, we performed array hybridization to determine changes in gene expression upon miR-101 overexpression in U2OS cells.

2015-05-22 | GSE69150 | GEO

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