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Repression of Cebpa-L1-AD-En2 enhancer decreases Cebpa expression in inguinal white adipose tissue and affects adipose tissue development

ABSTRACT: To investigate whether Cebpa-L1-AD-En2 produces a marked effect on adipogenesis in mouse adipose tissue, we injected the dCas9-KARB-En2 lentiviral system (iWAT_Cebpa-En2 group) or dCas9-KRAB-GFP (Control group) directly into mouse inguinal white adipose tissue (iWAT). We conducted RNA-seq analysis on the iWAT of iWAT-En2 group mice and control group mice at 13 weeks of age

ORGANISM(S): Mus musculus

PROVIDER: GSE229849 | GEO | 2024/03/07

REPOSITORIES: GEO

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Identifying functional enhancers regulating Cebpa expression and adipocyte differentiation (mRNA-Seq)

Project description:To explore the effect of enhancer repression on adipocyte differentiation in a transcriptome-wide manner, we infected cells with 1 mL lentiviruses medium containing dCas9-KRAB-En2-sgRNAs (Experimental group) or dCas9-KRAB (Control group) at the multiplicity of infection (MOI) of 100 for 12 h. We performed transcriptional profiling by RNA sequencing (RNA-seq) for dCas9-KRAB-En2 and dCas9-KRAB cells at day 7 of differentiation.

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Repression of Cebpa-L1-AD-En2 enhancer decreases Cebpa expression in inguinal white adipose tissue and affects adipose tissue development

Project description:Repression of Cebpa-L1-AD-En2 enhancer decreases Cebpa expression in inguinal white adipose tissue and affects adipose tissue development

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