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Effect of persistent ER stress on gene expression of pancreatic ductal adenocarcinoma cell AsPC1

ABSTRACT: To investigate the changes of gene expression in pancreatic ductal adenocarcinoma cells under persistent endoplasmic reticulum stress, we treated AsPC-1 cells with 30 nM thapsigargin (Tg) for more than 14 days (designated as AsPC-1-Tg). To investigate whether the effects of persistent ER stress on gene expression is permanent or reversible, we cultured AsPC-1-Tg cells in a medium without Tg (designated as AsPC-1-Tg-Re) for 4 days. In this study, we then performed gene expression profiling analysis using data obtained from high-throughput RNA-seq (include mRNA, lncRNA, circRNA and miRNA) of AsPC-1 (WT), AsPC-1-Tg (TG) and AsPC-1-Tg-Re (RE) cells. Three biological replicates of each sample were used for RNA-seq.

ORGANISM(S): Homo sapiens

PROVIDER: GSE229941 | GEO | 2023/08/09

REPOSITORIES: GEO

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