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Transciptional profile of Escherichia coli O157:H7 TUV93-0 inhibitor mutants in MEM-HEPES

ABSTRACT: Tpx, FolX and WrbA were identified as a targets for type 3 secretion inhibititors in pull-down assays.The transcriptional profile of Escherichia coli O157:H7 and isogenic mutants grown in MEM-HEPES were determined.

ORGANISM(S): Escherichia coli Escherichia coli O157:H7

PROVIDER: GSE23001 | GEO | 2011/07/05

SECONDARY ACCESSION(S): PRJNA129903

REPOSITORIES: GEO

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Transciptional profile of Escherichia coli O157:H7 TUV93-0 inhibitor mutants in MEM-HEPES

Project description:Tpx, FolX and WrbA were identified as a targets for type 3 secretion inhibititors in pull-down assays.The transcriptional profile of Escherichia coli O157:H7 and isogenic mutants grown in MEM-HEPES were determined. Total RNA from triplicate cultures growing exponentially in MEM-HEPES media were labelled with Cy5 (wild type) and Cy3 (tpxC61S). Labelled cDNAs were hybridised to UBEC3 arrays.

2011-07-05 | E-GEOD-23001 | biostudies-arrayexpress

Transcriptional analysis of Escherichia coli O157:H7 delta yedL in MEM-HEPES

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Transcriptional analysis of Escherichia coli O157:H7 delta yhaO in MEM-HEPES

Project description:Deletion of yhaO was found to signifcantly decrease type three secretion in EHEC O157:H7. Transcriptional profiles of Escherichia coli O157: H7 and the isogenic yhaO mutant were generated and compared.

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Transcriptional profile of pulsed AsxR in Escherichia coli O157:H7 str. TUV93-0

Project description:Transcript abundance in Escherichia coli O157:H7 was determined in the presence or absence of pulsed expression of the small RNA, AsxR. AsxR was cloned under the control the arabinose inducible promoter Para. Escherichia coli O157:H7 str. TUV93-0 with pAsxR or empty vector was cultured in MEM-HEPES media to an OD600 of 0.8 and 0.2% arabinose added. 10min after addition of arabinose 10ml of cells were harvested and and pellets resuspended in 1ml of Trizol and total RNA isolated. RNAs were labelled using the SuperScript Plus indirect cDNA labelling System. Triplicate control RNAs were pooled and hybridised to seperate AsxR test RNAs on three microarays. Arrays were hybridised using the Maui hybridisation platform and Scann using and Axon Autoloader Scanner. GenePix software was used to analyse images and GPR files were analysed using Genespring 7.3.1.

2014-05-22 | E-GEOD-46113 | biostudies-arrayexpress

Escherichia coli O157:H7

Project description:Transciptional profile of Escherichia coli O157:H7 TUV93-0 inhibitor mutants in MEM-HEPES

| PRJNA129903 | ENA

Transcriptional profile of pulsed AsxR in Escherichia coli O157:H7 str. TUV93-0

Project description:Transcript abundance in Escherichia coli O157:H7 was determined in the presence or absence of pulsed expression of the small RNA, AsxR.

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Escherichia coli O157:H7 isolate:O157:H7

Project description:Escherichia coli O157:H7 isolate:O157:H7 Genome sequencing

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Transcriptional response of Escherichia coli O157:H7 to cinnamaldehyde

Project description:Cinnamaldehyde is a natural antimicrobial and has been found to be effective against many foodborne pathogens including Escherichia coli O157:H7. Although its antimicrobial effects have been well investigated, limited information is available on its effects at the molecular level. Sublethal treatment at 200 mg/l cinnamaldehyde inhibited growth of E. coli O157:H7 at 37oC and for ≤ 2 h caused cell elongation, but from 2 to 4 h growth resumed and cells reverted to normal length. To understand this transient behaviour, genome-wide transcriptional analysis of E. coli O157:H7 was performed at 2 and 4 h exposure to cinnamaldehyde. Drastically different gene expression profiles were obtained at 2 and 4 h. At 2 h exposure, cinnamaldehyde induced overexpression of many oxidative stress-related genes, reduced DNA replication, and synthesis of protein, O-antigen and fimbriae. At 4 h, many cinnamaldehyde-induced repressive effects on E. coli O157:H7 gene expressions were reversed and oxidatve stress genes were nolonger differentially expressed.

2012-09-08 | GSE40693 | GEO

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2012-07-05 | GSE30424 | GEO

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