Bioprinting of Injectable Centimeter-scale Architectures with a Visible-light Crosslinking Bioink

ABSTRACT: This study propose a novel visible-light crosslinking hydrogel bioink (termed GP) to fabricate injectable centimeter-scale architectures. Composed of GelMA with large molecular weight PEGDA, GP can form a reinforced dual-crosslinking network, presenting excellent superelasticity and stability, supporting injection pass nozzles with 1.5mm inner diameter after bioprinted as 15mm×15mm scale architectures. Moreover, when printing with living cells, even for sensitive cell lines like genome-edited cells, the transcriptome of cells after printing shows no difference, suggesting the high cell printing suitability of GP and the visible-light extrusion printing technique. The printed architectures can support long-term culture for at least 14 days, and GP provides cells with a biocompatible microenvironment, supporting cell survival, proliferation, and function maintenance.

ORGANISM(S): Homo sapiens

PROVIDER: GSE230068 | GEO | 2023/10/19

REPOSITORIES: GEO