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Effect of visible light on human skin fibroblasts

ABSTRACT: Effect of visible light on human skin fibroblasts

PROVIDER: PRJNA448517 | ENA |

REPOSITORIES: ENA

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Effect of visible light on human skin fibroblasts

Project description:Artificial visible light is everywhere in our modern life. Our mode of social communication confronts us with screens of all kinds and their use is on the rise. People are therefore increasingly exposed to artificial visible light of which effects on skin are still largely poorly known. The purpose of this study was to model the artificial visible light emitted by electronic devices and subsequently assess the effect of such a light in normal human fibroblasts.

2018-06-30 | GSE112614 | GEO

RNA-seq in normal human dermal fibroblast cells for understanding protective effects of visible red light (660nm) in response to UVB (312nm) exposure

Project description:The aim of this study is identifying potential signaling pathways involve with visible red light induced photoprotective effect against skin damage by UVB exposure, using transcriptomic analysis

2021-06-20 | GSE116968 | GEO

Next Generation Sequencing to identify the expression of transcripts and abnormal events in 3D skin exposed to blue wavelength and full spectrum of visible light

Project description:Samples of 3D skin, irradiated using LED light and compared with un-exposed control, regarding one- and four-days of incubation. Three groups were simulating acute exposure: 1h, 2h and 4 hours whereas the 3D skin samples irradiated for 1 hour over four sequential days were simulating repeated exposure, for both blue wavelength and the full visible spectrum of digital light.

2023-11-28 | GSE190106 | GEO

Effect of visible light on the transcriptome of Clostridium autoethanogenum during gas fermentation

Project description:The expression profile of C. autoethanogenum DSM 10061 grown autotrophically with H2:CO:CO2 under visible light at an intensity of 4200 lux versus the expression profile of C. autoethanogenum DSM 10061 grown autotrophically in the dark

2023-07-01 | GSE207468 | GEO

Effect of visible light on the transcriptome of Clostridium autoethanogenum during gas fermentation

Project description:Effect of visible light on the transcriptome of Clostridium autoethanogenum during gas fermentation

| PRJNA855865 | ENA

Bioprinting of Injectable Centimeter-scale Architectures with a Visible-light Crosslinking Bioink

Project description:This study propose a novel visible-light crosslinking hydrogel bioink (termed GP) to fabricate injectable centimeter-scale architectures. Composed of GelMA with large molecular weight PEGDA, GP can form a reinforced dual-crosslinking network, presenting excellent superelasticity and stability, supporting injection pass nozzles with 1.5mm inner diameter after bioprinted as 15mm×15mm scale architectures. Moreover, when printing with living cells, even for sensitive cell lines like genome-edited cells, the transcriptome of cells after printing shows no difference, suggesting the high cell printing suitability of GP and the visible-light extrusion printing technique. The printed architectures can support long-term culture for at least 14 days, and GP provides cells with a biocompatible microenvironment, supporting cell survival, proliferation, and function maintenance.

2023-10-19 | GSE230068 | GEO

Bioprinting of Injectable Centimeter-scale Architectures with a Visible-light Crosslinking Bioink

Project description:Bioprinting of Injectable Centimeter-scale Architectures with a Visible-light Crosslinking Bioink

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Effect of cell harvesting technique and storage on metabolic profiles in human skin fibroblasts

Project description:Human skin fibroblasts were cultured in MEM media supplemented with 15% FBS. Cells were harvested using (i) trypsinization, (ii) scraping, (iii) methanol fixation and scraping, (iV) methanol fixation, scraping, and drying. Targeted metabolomics analysis of organic acids, amino acids, and acycarnitines was conducted at Mayo Clinic Metabolomics Core Facility.

2019-02-27 | ST001148 | MetabolomicsWorkbench

Mice Skin Fibroblasts Proteome after E3 ubiquitin ligases KLHL24 overactivated

Project description:Skin fibroblasts were collected from WT and K24KI (Klhl24c.3G>T knock-in) mice by FACS to explore the protein changes in K24KI mice.

2022-11-15 | PXD038166 |

Whole-genome microarray analysis of human skin fibroblasts

Project description:Connective tissue growthfactor (CTGF/CCN2) is a matricellular protein induced by transforming growth factor-beta (TGF-beta) and intimately involved with tissue repair and overexpressed in various fibrotic conditions. We have previously shown that keratinocytes in vitro downregulate TGF-beta induced expression of CTGF in fibroblasts by an interleukin-1 alfa (IL-1alfa) dependent mechanism. With this study we want to get a better overall perspective who fibroblasts respond on TGF-beta and in a TGF-Beta stinulated system, what effect addition of IL-1 alfa have. This to understand the expression of CTGF in human fibroblasts which in turn have implications for the pathogenesis of fibrotic conditions involving the skin. Microarray was performed on human skin fibroblast RNA from one patient, Fibroblasts were seeded in vitro as a control (Control, C), TGF-beta were added to fibroblasts and incubated for 16 h (addition of TGF-beta, T) and both IL-1alfa and TGF-beta were added to fibroblasts for 16 h (addition of IL-1alfa and TGF-beta, IT). Each condition (C,T and IT) were done in three replicates.

2016-04-14 | E-GEOD-69447 | biostudies-arrayexpress

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