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A gut commensal protist protects against virus-mediated loss of oral tolerance

ABSTRACT: To better understand how Tritrichomonas arnold colonization impacts the reovirus-mediated proinflammatory response in dietary antigen-presenting dendtric cells (cDC1), we examined the transcriptional profile of mesenteric lymph node-derived cDC1. For RNA-sequencing on CD103+ CD11b- CD8a+ DCs, 1,000 CD103+ CD11b- CD8a+ DCs were FACS sorted into a 96-well plate

ORGANISM(S): Mus musculus

PROVIDER: GSE230530 | GEO | 2023/06/07

REPOSITORIES: GEO

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A gut commensal protist protects against virus-mediated loss of oral tolerance_2

Project description:To better understand how Tritrichomonas arnold colonization impacts the reovirus-mediated proinflammatory response in mesenteric lymph nodes, we examined the transcriptional profile of mesenteric lymph nodes For RNA-sequencing single cell suspension of mesenteric lymph nodes were lysed in RLT buffer (Qiagen) and RNA was isolated

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