Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from human foreskin fibroblasts (HFFs) infected with Toxoplasma gondii

ABSTRACT: Through targeted CRISPR screening of the Toxoplasma gondii secretome, we identified GRA57 as a novel secreted effector required for survival in interferon-gamma (IFNg)-activated human foreskin fibroblasts (HFFs). In this experiment we aimed to determine if GRA57 affects the host cell transcriptional response to Toxoplasma, and also whether deletion of GRA57 affects the parasite transcriptome.

ORGANISM(S): Homo sapiens

PROVIDER: GSE230866 | GEO | 2023/05/27

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression data from human foreskin fibroblasts (HFFs) infected with Toxoplasma gondii

Project description:Expression data from human foreskin fibroblasts (HFFs) infected with Toxoplasma gondii

| PRJNA962881 | ENA

Expression data from host cells infected with different strains of Toxoplasma gondii

Project description:This SuperSeries is composed of the following subset Series: GSE25468: Expression data from Human foreskin fibroblasts (HFFs) infected with Toxoplasma gondii. GSE25469: Expression data from WT or p65-/- mouse embryonic fibroblasts (MEFs) infected with Toxoplasma gondii. Refer to individual Series

2011-01-10 | E-GEOD-25476 | biostudies-arrayexpress

Expression data from human foreskin fibroblasts (HFFs) infected with type I strains of Toxoplasma gondii.

Project description:Type I strains of Toxoplasma gondii exhibit phenotypic variation, but it is uncertain how differently type I strains modulate the host cell. We determined differential host modulation by type I strains through microarray. HFFs were infected with RH-ERP, RH-JSR and GT1 for 24 hours. Total RNA was isolated and hybridized to Affymetrix GeneChip Human Genome U133A 2.0 arrays.

2013-08-20 | E-GEOD-44189 | biostudies-arrayexpress

Expression data from cyclohexamide treated Human Foreskin Fibroblasts (HFFs) infected with type I Toxoplasma gondii and stimulated with interferon gamma.

Project description:Pre-infection with Toxoplasma parasites inhibits induction of an interferon gamma responsive gene expression program. In this study we aimed to determine what role host negative regulatory proteins that are induced by Toxoplasma infection, such as SOCS-1, have in this inhibition. We used Affymetrix microarrays to analyze host cell transcription after pre-treatment with the protein synthesis inhibitor cyclohexamide (CHX), Toxoplasma infection, and subsequent interferon-gamma stimulation. Human foreskin fibroblasts (HFFs) were left untreated or treated with CHX for 40min. Cells were infected, or not, with type I (RH) Toxoplasma parasites, for one hour, and subsequently stimulated with human IFN-γ for one hour. Total RNA was isolated and hybridized to Affymetrix Human U133A 2.0 gene chips.

2015-08-01 | E-GEOD-34916 | biostudies-arrayexpress

Effect of Toxoplasma gondii efector TgIST on global transcriptome of human foreskin fibroblasts (HFFs) upon type I IFN activation

Project description:TgIST is an effector secreted by Toxoplasma gondii into the host cell that translocates to the nucleus and block STAT1 mediated transcription. STAT1 is involved in type I and II interferon (IFN) mediated upregulation of range of anti-parasitic molecules. A total of 544,234,059 read sequences generated from 3 independent biological replicates were mapped to human hg19 reference genome. Type I IFN activation of HFFs infected with TgIST knockout parasites showed upregulation of genes involved in interferon signaling compared to wild type parasites.

2019-06-30 | GSE125066 | GEO

Transcription profiling by array of human foreskin fibroblasts infected with different Toxoplasma gondii strains

Project description:Effect of different Toxoplasma gondii strains on human foreskin fibroblast gene expression

2007-01-18 | E-MEXP-783 | biostudies-arrayexpress

Expression data from Human foreskin fibroblasts (HFFs) infected with Toxoplasma gondii.

Project description:Toxoplasma strains have been shown to modulate host cell transcription. We have found a type II Toxoplasma gene, GRA15, which activates the nuclear translocation of the NF-kappaB p65 transcription factor. We used microarrays to determine how GRA15II modulates host cell transcription. HFFs were infected with type I (RH), type I GRA15II (RH GRA15II), type II (Pru), type II GRA15KO (Pru GRA15KO), type III (cep), or type III GRA15II (cep GRA15II) parasites for 18-24 hours. Some samples were also stimulated with TNF-alpha. Total RNA was isolated and hybridized to Affymetrix GeneChip Human Genome U133A 2.0 arrays.

2011-01-10 | E-GEOD-25468 | biostudies-arrayexpress

Expression data from human foreskin fibroblasts (HFFs) infected with type I strains of Toxoplasma gondii.

2013-08-20 | GSE44189 | GEO

HFF Cells: Control vs. Toxoplasma gondii infected

Project description:Expression profile microarray of human foreskin fibroblast cell comparing control untreated HFF cell with HFF cell infected with ME49 strain.Study on Toxoplasma gondii infection of HFF cell LncRNAs expression, for further studies on the differential exprssion of LncRNAs in HFF cell against the infection of Toxoplasma gondii research provide the basic function.

2017-01-01 | GSE92603 | GEO

Genome-wide analysis of gene expression response upon infection with Toxoplasma gondii

Project description:The in vitro effect of infection with different strains of Toxoplasma gondii was tested 24 hours after infection of Human Foreskin Fibroblasts (HFF) The strains tested include RH, VEG, and transgenic strains of RH overexpressing ROP38 or ROP21 Total RNA of Toxoplasma gondii infected HFF cell was compared to uninfected cells

2010-07-31 | E-GEOD-22402 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data