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CPSF6 regulates alternative polyadenylation and proliferation of cancer cell through phase separation

ABSTRACT: In order to explore the impact of phase separation of CPSF6 on APA, we identified the structural domains that affect its phase separation formation and performed mutations. In addition, we also found that the phosphorylation of CPSF6 can also affect its phase separation effect, and identified key kinases. We performed IVT-SAPAS sequencing on both cases mentioned above.

ORGANISM(S): Homo sapiens

PROVIDER: GSE231823 | GEO | 2023/09/03

REPOSITORIES: GEO

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CPSF6 regulates alternative polydenylation and proliferation of cancer cell through phase separation Ⅱ

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CPSF6 regulates alternative polyadenylation and proliferation of cancer cell through phase separation

Project description:CPSF6 regulates alternative polyadenylation and proliferation of cancer cell through phase separation

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Phosphorylation of CPSF6 RS-like domain

Project description:Cleavage factor I mammalian (CFIm) complex, composed of cleavage and polyadenylation specificity factor CPSF6, regulates alternative polyadenylation (APA). CPSF6 has a RS-like domain which plays role in protein -protein interactions. This interaction might have role in alternative polyadenylation site selection. The phosphorylation of RS- like domain might play role in protein -protein interaction and thus might have a role in alternative polyadenylation site selection. So we did mass specteroanalysis to analyse phosphorylation sites of RS-like domain of CPSF6.

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