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Capture Hi-C in (EV and ZFP661-3HA OE) mESCs and E16.5 mouse forebrains

ABSTRACT: This assay aimed to investigate the regulatory role of ZFP661 in modulating the permeability of CTCF barriers. To achieve this, we generated Capture Hi-C maps on several regions bound by ZFP661 using E16.5 forebrains from wild-type (WT) and Zfp661 knockout (KO) mice, as well as empty vector (EV) control and ZFP661-3HA overexpressing (OE) mESCs. Our findings reveal that ZFP661 modulates the permeability of CTCF barriers, thereby balancing between the interactions that are trapped at the barriers and those passing over them.

ORGANISM(S): Mus musculus

PROVIDER: GSE231864 | GEO | 2023/05/09

REPOSITORIES: GEO

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