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Itaconate enhances oncolytic virotherapy via multitarget inhibition of antiviral and inflammatory pathways

ABSTRACT: 4 octyl itaconate (4-OI) is a known anti-inflammatory chemical that activates Nrf2 signaling. Here, we are exploring the capacities of 4-OI to promote the spread of oncolytic Vesicular Stomatitis Virus (VSVd51M) in pLenti control kidney adenocarcinoma 786-O cell line and in Nrf2 knock out 786-O.

ORGANISM(S): Homo sapiens

PROVIDER: GSE232509 | GEO | 2023/06/15

REPOSITORIES: GEO

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Effect of Irg1-Itaconate on Nrf2-transcripting genes in macrophages by using chromatin immunoprecipitation-sequencing (ChIP-seq) assays.

Project description:To investigate the role of Irg1-itacaonate on Nrf2-transcripting genes in macrophages, RAW264.7 cells were cultured and were treated 120 μM 4-octyl itaconate (4-OI) or Vehicle for 8 h. We then performed ChIP-seq analysis from 4-OI or vehicle treated RAW264.7 cells using Nrf2 or IgG antibodies.

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4-octyl itaconate inhibits aerobic glycolysis by targeting GAPDH to exert anti-inflammatory effects

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Expression data from PBMCs infected with influenza A virus and treated with/without Itaconate

Project description:Analysis of gene expression in human PBMCs infected with influenza A virus or Mock and treated with Itaconic acid, Dimethyl itaconate or 4-Octyl itaconate to investigate the effects itaconate have on transcriptional response in human PBMCs. We particularly looked at anti-inflammatory effects of itaconate in inflammation followed by Influenza A virus infection.

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