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Single Cell RNA sequencing of the Monocytes from controls and pulmonary arterial hypertension (PAH) patients

ABSTRACT: We report the application of single cell RNA sequencing on human Peripheral Blood Mononuclear cells. The 12 samples are sequenced as biological replications (6 control and 6 patients), and pooled at the analysis stage. The library was prepared using the Chromium Single Cell 3' v2 and v3 Reagent Kit, and sample processing was under bcl2fastq2/2.20.0 and cellranger/2.2.0. We report a decreased CD14 in classical monocytes among PAH patients, with greater potential of apoptosis for CD14+ monocytes population. This study provide a reference for studying the monocytes related abonormalties in PAH and other inflammatory diseases.

ORGANISM(S): Homo sapiens

PROVIDER: GSE233189 | GEO | 2023/06/01

REPOSITORIES: GEO

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