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Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis [ATAC-seq]

ABSTRACT: To establish a new model system with which to study cerebellar granule lienage development and disease, human hbNES cells were differentiated to cerebellar granule neurons over a 56 day period.

ORGANISM(S): Homo sapiens

PROVIDER: GSE233336 | GEO | 2023/05/29

REPOSITORIES: GEO

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Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis [scRNA-seq]

Project description:To establish a new model system with which to study cerebellar granule lienage development and disease, human hbNES cells were differentiated to cerebellar granule neurons over a 56 day period.

2023-05-29 | GSE233338 | GEO

Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis [RNA-seq]

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Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis.

Project description:Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis.

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Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis [scRNA-seq]

Project description:Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis [scRNA-seq]

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Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis [RNA-seq]

Project description:Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis [RNA-seq]

| PRJNA976053 | ENA

Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis [ATAC-seq]

Project description:Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis [ATAC-seq]

| PRJNA976054 | ENA

Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis.

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-05-29 | GSE233339 | GEO

Gene expression of cerebellar granule cell layer

Project description:We performed gene-expression analysis of mouse cerebellar granule cell layer as compared to that of Purkinje cells. DNA microarray analysis detected genes in cerebellar granule cell layer, most of which are classified into functional molecule categories. Our comparative analysis between Purkinje cells and the granule cell layer showed that the characteristic expression pattern in Purkinje cells was particularly represented by M-bM-^@M-^\the neural communication systemM-bM-^@M-^] components. Pukinje cells and granule cell layer of the mouse cerebellum were collected by laser microdissection for RNA extraction and hybridization on Affymetrix microarrays.

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Notch signaling between cerebellar granule cell progenitors

Project description:During cerebellar development, maternal granule cell progenitors (GCPs) divide to produce not only postmitotic granule cells (GCs) but also sister GCPs. However, molecular machinery to proportionally produce distinct sister cell types from seemingly uniform GCPs is still elusive. Here we report Notch signaling makes a difference among GCPs, leading to their proportional differentiation.

2020-06-27 | GSE153313 | GEO

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