Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

UHRF1 is a mediator of KRAS driven oncogenesis in lung adenocarcinoma [CRISPR_screen]

ABSTRACT: We performed a focused CRISPR in KRAS mutant lung cancer cells (A549) using a minipool sgRNA library targeting 80 lung cancer specific tumor suppressor genes and 12 control genes. This screen identified tumor suppressor genes regulated by Uhrf1 in KRAS mutant lung cancer.

ORGANISM(S): Homo sapiens

PROVIDER: GSE233401 | GEO | 2023/05/25

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

UHRF1 is a mediator of KRAS driven oncogenesis in lung adenocarcinoma [RNA-seq]

Project description:RNA sequening was performed to understand the role of UHRF1 in regulation of gene expression in KRAS mutant lung cancer cell lines. Knock-down of both UHRF1 and KRAS leads to significant and overlapping changes in gene expression in both cell lines.

2023-05-15 | GSE198289 | GEO

UHRF1 is a mediator of KRAS driven oncogenesis in lung adenocarcinoma [epic_methyl]

Project description:Methylation array was performed to understand the role of UHRF1 in DNA methylation in KRAS mutant lung cancer cell lines. Knock-down of UHRF1 leads to significant changes in global DNA methylation in both cell lines.

2023-05-15 | GSE198446 | GEO

UHRF1 is a mediator of KRAS driven oncogenesis in lung adenocarcinoma [CRISPR_screen]

Project description:UHRF1 is a mediator of KRAS driven oncogenesis in lung adenocarcinoma [CRISPR_screen]

| PRJNA976201 | ENA

UHRF1 is a mediator of KRAS driven oncogenesis in lung adenocarcinoma [shRNA]

Project description:Using pooled shRNA libraries we performed a focused screen of 115 genes in 3D ex vivo cultures of primary murine lung tumor cells and 2D cultures of murine LKR10 cells. These screens identify Uhrf1 as gene selectively important for the 3D growth of primary lung cancer spheroids.

2023-05-15 | GSE209923 | GEO

UHRF1 is a mediator of KRAS driven oncogenesis in lung adenocarcinoma

Project description:UHRF1 is a mediator of KRAS driven oncogenesis in lung adenocarcinoma

| PRJNA815323 | ENA

UHRF1 is a mediator of KRAS driven oncogenesis in lung adenocarcinoma [epic_methyl]

Project description:UHRF1 is a mediator of KRAS driven oncogenesis in lung adenocarcinoma [epic_methyl]

| PRJNA815313 | ENA

UHRF1 is a mediator of KRAS driven oncogenesis in lung adenocarcinoma [shRNA]

Project description:UHRF1 is a mediator of KRAS driven oncogenesis in lung adenocarcinoma [shRNA]

| PRJNA862950 | ENA

UHRF1 is a mediator of KRAS driven oncogenesis in lung adenocarcinoma [RNA-seq]

Project description:UHRF1 is a mediator of KRAS driven oncogenesis in lung adenocarcinoma [RNA-seq]

| PRJNA814495 | ENA

Transcriptome analysis to UHRF1 depletion in RaeL cells

Project description:RNAseq was used to identify host and viral transcriptome changes in UHRF1 knock-out RaeL cells. UHRF1 KO RaeL cells were subjected to FACSort for ICAM1+ subpopulations, which were further used for RNAseq analysis. The RaeL cells expressing control sgRNA was used as the control.

2020-05-04 | GSE136596 | GEO

Gene expression profiles of uhrf1 mutant zebrafish

Project description:UHRF1 (Ubiquitin-like, containing PHD and RING finger domains, 1) recruits DNMT1 to hemimethylated DNA during replication, is essential for maintaining DNA methylation patterns during cell division and is suggested to direct additional repressive epigenetic marks. Uhrf1 mutation in zebrafish results in multiple embryonic defects including failed hepatic outgrowth, but the epigenetic basis of these phenotypes is not known. We find that DNA methylation is the only epigenetic mark that is depleted in uhrf1 mutants and make the surprising finding that despite the reduced organ size in uhrf1 mutants, genes regulating DNA replication and S-phase progression were highly upregulated. Further, there is a striking increase in BrdU incorporation in uhrf1 mutant cells, and they retained BrdU labeling over several days, indicating they are arrested in S-phase. Moreover, some of the label retaining nuclei co-localized with TUNEL positive nuclei, suggesting that arrested cells are responsible for apoptosis. Importantly, dnmt1 mutation phenocopies the S-phase arrest and hepatic outgrowth defects in uhrf1 mutants and Dnmt1 knock-down enhances the uhrf1 hepatic phenotype. Together, these data indicate that DNA hypomethylation is sufficient to generate the uhrf1 mutant phenotype by promoting an S-phase arrest. We thus propose that cell cycle arrest is a mechanism to restrict propagation of epigenetically deranged cells during embryogenesis. Genome-wide expression profiling was performed on 2 uhrf1 mutant and 2 wildtype zebrafish larvae (120 hours post fertilization) by using Zebrafish Genome Array (Affymetrix) according to manufacturer's instruction.

2015-02-09 | E-GEOD-55339 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data