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Effect of combinatorial transcription factor overexpression on iPSC-derived endothelial cells

ABSTRACT: We aimed to investigate how transcription factors alter brain-specific and zonation-identity of endothelial cells. We used a dox-inducible lentivirus to increase expression of specific combinations of transcription factors expressed by human brain endothelial cells in iPSC-derived endothelial cell (iEC) monolayers. Using SMART-seq we compared gene expression profiles and benchmarked gene expression to iPSC-derived brain microvascular endothelial-like cells (iBMECs).

ORGANISM(S): Homo sapiens

PROVIDER: GSE233694 | GEO | 2023/06/06

REPOSITORIES: GEO

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