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Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures.

ABSTRACT: The ability of sister blastomeres of 8-cell mouse embryos to produce ESC lines was measured, and the transcriptome of the Bm-mESCs lines generated was analyzed by RNA-seq.

ORGANISM(S): Mus musculus

PROVIDER: GSE233762 | GEO | 2023/10/17

REPOSITORIES: GEO

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Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures.

Project description:Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures.

| PRJNA977721 | ENA

Single cell analysis of sister blastomeres of parthenogenetic 2-cell mouse embryos respecting pair associations

Project description:Following fertilization in mammals, it is generally accepted that totipotent cells are exclusive to the zygote and to each of the two blastomeres originating from the first mitotic division. We counter that this classic view needs to be revised, because we have presented compelling evidence that the sister blastomeres are both totipotent in only a subset of 2-cell stage mouse embryos (PMID 28811525). Building on our previous findings, we here ask the question if the interblastomere differences depend - at least in part - on the contribution of sperm, since the area of sperm entry is inherited preferentially by one blastomere. To this end, we created sister 2-cell stage blastomeres without sperm entry point, by parthenogenesis. We compare the transcriptomes of the sister blastomeres, to see if the interblastomere mRNA differences observed after fertilization (GSE94050) are still there when the sperm entry point is missing.

2019-07-10 | GSE111589 | GEO

Single cell analysis of sister blastomeres of 2-cell mouse embryos respecting pair associations

Project description:Upon 2-cell embryo splitting, individual blastomeres were compared and contrasted with each other respecting pair associations (e.g. blastomere '1a' and '1b' of embryo 1, '2a' and '2b' of embryo 2, and so forth) Transcriptome analysis followed by cluster analysis (Ward) was able to match a minority of the blastomeres with the correct sister blastomere

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Transcript profiling of individual twin blastomeres derived by splitting 2-cell stage murine embryos

Project description:We have examined whether twin blastomeres from 2-cell stage mouse embryos differ in mRNA content. Amplified mRNA from 12 blastomeres derived from six embryos approximately mid-way through their second cell cycle was analyzed. Probes displaying normalized values greater than 0.25 were selected and examined for consistent bias in expression within blastomere pairs. Although transcript content varied both between individual embryos and twin blastomeres, no consistent asymmetries were observed for the majority of genes. On the other hand, 769 genes displayed a greater than 1.4-fold difference in expression across 5 of 6 pairs of blastomeres and 178 genes differed across all 6 pairs. These genes separated into two groups by class discovery clustering. Of the 769 differentially expressed genes, 163 were significantly up- or down-regulated in one sister blastomere compared to the other. Transcripts encoding proteins implicated in RNA processing and cytoskeletal organization were highly represented among the most abundant, differentially distributed mRNA. We conclude that there are many differences that distinguish twin blastomeres derived from a single 2-cell stage embryo but that only a few of these differences are consistent across multiple pairs of embryos. We hypothesize that a stochastically-based lack of synchrony in cell cycle progression between the two cells might explain some or all of the asymmetries in transcript composition. 6 pairs of blastomeres were analyzed for a total of 12 samples.

2010-05-28 | E-GEOD-21688 | biostudies-arrayexpress

Transcript profiling of individual twin blastomeres derived by splitting 2-cell stage murine embryos

2010-05-15 | GSE21688 | GEO

Human Stem Cells from Single Blastomeres Reveal Pathways of Embryonic or Trophoblast Fate Specification.

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The Aryl Hydrocarbon Receptor Directs the Differentiation of Progenitor Blastomeres

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Gene expression profiles of individual blastomeres of early Ciona embryos

Project description:We examined gene expression profiles of individual blastomeres of early Ciona embryos using microarrays.

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Comparison of animal-blastomeres' RNA content to vegetal-most half of vegetal blastomeres' RNA content at the 8-cell stage of Xenopus laevis embryos to identify animal-enriched transcripts

Project description:In Xenopus laevis, a number of studies identified vegetal factors that specify the germ line, endoderm and dorsal axis, but there are few studies demonstrating roles for animal-enriched maternal mRNAs. Therefore, we carried out a microarray analysis to identify novel maternal transcripts enriched in animal blastomeres. We sought to maximize differences between animal and vegetal samples. To that end, we dissected 8-cell embryos into animal blastomeres and vegetal blastomeres, and further dissected the vegetal blastomeres into vegetal-most halves (VP) and equatorial regions (discarded).

2013-12-21 | E-GEOD-48659 | biostudies-arrayexpress

Single cell analysis of sister blastomeres of parthenogenetic 2-cell mouse embryos respecting pair associations

Project description:Single cell analysis of sister blastomeres of parthenogenetic 2-cell mouse embryos respecting pair associations

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