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Antibody-free fluorine assisted metabolic sequencing of RNA N4-acetylcytidine

ABSTRACT: More researches have revealed that N4-acetylcytidine (ac4C) affected a variety of cellular and biological processes. In order to better understand the ac4C roles in biology and disease, we present an antibody-free, fluorine assisted metabolic sequencing method to detect RNA N4-acetylcytidine, called ‘FAM-seq’. We have successfully applied FAM-seq to profile ac4C landscapes in humans. By comparing with the classic ac4C antibody sequencing method, we demonstrated that FAM-seq is a convenient and specific method for transcriptome-wide detection of ac4C. This method holds promise to detect nascent RNA ac4C modifications.

ORGANISM(S): Homo sapiens

PROVIDER: GSE234988 | GEO | 2023/10/02

REPOSITORIES: GEO

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N4-acetylcytidine of Nop2 mRNA is Required to the Transition of Morula-to-Blastocyst

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Antibody-free fluorine assisted metabolic sequencing of RNA N4-acetylcytidine

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