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Ruler Arrays Reveal Haploid Genomic Structural Variation

ABSTRACT: A ruler array combines novel sample preparation protocols, tiling genomic microarrays, and a new computational method to turn each probe on a microarray into a ruler that measures the physical distance between defined genomic sequences regardless of the intervening sequence. The ruler array protocol involves (1) genomic DNA purification (2) digestion with a restriction enzyme (3) ligation of a biotinylated adapter molecule to the cut sites (4) purification on streptavidin beads (5) polymerase extensions from a primer complementary to the adapter using labeled dNTPs and (6) hybridization to a tiling microarray

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE23524 | GEO | 2010/09/16

SECONDARY ACCESSION(S): PRJNA131777

REPOSITORIES: GEO

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Ruler Arrays Reveal Haploid Genomic Structural Variation

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