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Adipose, dermis and neural embryonic stem cells

ABSTRACT: The objective is to study gene expression between potential adult stem cells isolated from dermis and adipose tissue compared to embryonic neural stem cells. Tissue was enzymatically digested and cells were seeded into a tissue culture flask. After 4 days, cells that had grown in suspension were passaged, harvested (by sedimentation, mechanically triturated and replated. Seven days later, cells were passaged again and RNA isolated after another 7 days. Keywords: other

ORGANISM(S): Mus musculus

PROVIDER: GSE2353 | GEO | 2005/03/03

SECONDARY ACCESSION(S): PRJNA91649

REPOSITORIES: GEO

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Transcription profiling of mouse adipose, dermis and neural embryonic cells

Project description:The objective is to study gene expression between potential adult stem cells isolated from dermis and adipose tissue compared to embryonic neural stem cells. Tissue was enzymatically digested and cells were seeded into a tissue culture flask. After 4 days, cells that had grown in suspension were passaged, harvested (by sedimentation, mechanically triturated and replated. Seven days later, cells were passaged again and RNA isolated after another 7 days.

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