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Host pathogen interaction between Staphylococcus aureus ATCC25923 and murine RAW264.7 macrophage-like cells

ABSTRACT: Macrophages infected with S. aureus were subjected to gene expression profiling to undertake a complete understanding of the interaction induced gene expression changes in both, S.aureus and the RAW macrophages.

ORGANISM(S): Mus musculus Staphylococcus aureus

PROVIDER: GSE23538 | GEO | 2010/08/11

SECONDARY ACCESSION(S): PRJNA131749

REPOSITORIES: GEO

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Host pathogen interaction between Staphylococcus aureus ATCC25923 and murine RAW264.7 macrophage-like cells

Project description:Macrophages infected with S. aureus were subjected to gene expression profiling to undertake a complete understanding of the interaction induced gene expression changes in both, S.aureus and the RAW macrophages. Agilent one-color experiment, Agilent-021933 Genotypic designed Custom Staphylococcus aureus and Mus musculus 8x15k

2010-08-10 | E-GEOD-23538 | biostudies-arrayexpress

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Project description:MepR is a substrate-responsive repressor of mepR and mepA, which encode itself and a MATE family multidrug efflux pump. Microarray analyses of Staphylococcus aureus SH1000 and its mepR-disrupted derivative revealed changes in expression of many genes in addition to mepR and mepA, notably several involved in virulence Keywords: Staphylococcus aureus, MATE efflux pump, MepR Staphylococcus aureus strains SH1000 wildtype and mepR were grown in duplicate to exponential and post-exponential phase (corresponding to an A550 nm of 0/4 and 2.0 respectively). RNA was harvested, converted to cDNA, labelled with Biotin and used to probe custom-designed Affymetrix antisense S.aureus GeneChips. Eight samples in total were prepared and analyzed.

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