Proteomic and metabolomic profiling of methicillin resistant and methicillin sensitive Staphylococcus aureus using a simultaneous extraction protocol

ABSTRACT: understanding the biology of methicillin resistant staphylococcus aureus (MRSA) is crucialto unlocking insights for new targets in the fight against this pathogen, there is howeverlimited reports of methadological approaches for carrying proteomic and metabolomic profiling in S.aureus. Therefore, we describe the use of a dual-functionality methanol extraction method for the concurrent extraction of protein and metabolites from S.aureus and reporton the comparative analysis of the proteomic and metabolomic profiles of MRSA versus methicillin sensitive S. aureus (MSSA). Using a reference strain from MRSA and MSSA, we first compared the MRSA proteome extracted using the methanol method to the one from the traditionally used urea method. Then using the methanol extraction method, we compared the proteome and metabolome of MRSA versus MSSA. Through this study, we demonstrated the effectivnessof the methanol-based-dual-extraction method, providing simultaneous insights into the proteomic and metabolomic landscapes of S.aureus strains, demonstrating the utility of proteomic and metabolomic profiling for elucidating the biological basis of antimicrobial resistance

INSTRUMENT(S): timsTOF

ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Core Facility  

LAB HEAD: Abiola Senok

PROVIDER: PXD050411 | Pride | 2024-08-10

REPOSITORIES: Pride