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Juvenile rainbow trout exposed in situ at site receiving different concentrations of municipal wastewater effluent (MWWE) and an upstream reference site

ABSTRACT: Juvenile rainbow trout were exposed to MWWE in a caging study, with cages held upstream and downstream of a MWWE outfall for two weeks in the fall of 2007. Whole tissues were sampled and analyzed. Microarray analysis was conducted on liver samples.

ORGANISM(S): Oncorhynchus mykiss

PROVIDER: GSE23613 | GEO | 2011/10/07

SECONDARY ACCESSION(S): PRJNA129957

REPOSITORIES: GEO

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Project description:BackgroundIn the United States, an estimated 14,748 wastewater treatment plants (WWTPs) provide wastewater collection, treatment, and disposal service to more than 230 million people. The quality of treated wastewater is often assessed by the presence or absence of fecal indicator bacteria. UV disinfection of wastewater is a common final treatment step used by many wastewater treatment plants in order to reduce fecal coliform bacteria and other pathogens; however, its potential impacts on the total effluent bacterial community are seemingly varied. This is especially important given that urban WWTPs typically return treated effluent to coastal and riverine environments and thus are a major source of microorganisms, genes, and chemical compounds to these systems. Following rainfall, stormflow conditions can result in substantial increases to effluent flow into combined systems.MethodsHere, we conducted a lab-scale UV disinfection on WWTP effluent using UV dosage of 100 mJ/cm2 and monitored the active microbiome in UV-treated effluent and untreated effluent over the course of 48 h post-exposure using 16S rRNA sequencing. In addition, we simulated stormflow conditions with effluent UV-treated and untreated effluent additions to river water and compared the microbial communities to those in baseflow river water. We also tracked the functional profiles of genes involved in tetracycline resistance (tetW) and nitrification (amoA) in these microcosms using RT-qPCR.ResultsWe showed that while some organisms, such as members of the Bacteroidetes, are inhibited by UV disinfection and overall diversity of the microbial community decreases following treatment, many organisms not only survive, but remain active. These include common WWTP-derived organisms such as Comamonadaceae and Pseudomonas. When combined with river water to mimic stormflow conditions, these organisms can persist in the environment and potentially enhance microbial functions such as nitrification and antibiotic resistance.

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Juvenile rainbow trout exposed in situ at site receiving different concentrations of municipal wastewater effluent (MWWE) and an upstream reference site

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