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Structural basis of nucleosomal H4K20 recognition and methylation by SUV420H1 methyltransferase

ABSTRACT: Here we report the cryo-electron microscopy (cryo-EM) structures of SUV420H1 associated with canonical nucleosome core particles (NCPs) or H2AZ containing NCPs. We find that SUV420H1 shows conformational change after bound to a nucleosome and make extensive site-specific contacts with histone and DNA regions, thus enabling H4K20 insertion for catalysis specifically. Through in vivo functional analysis, we validated special residues of SUV420H1 that are critical for its catalytic activity and function in chromatin state regulation.Thus, our study provides molecular insights into the nucleosome-based recognition and histone- methylation mechanisms of SUV420H1.

ORGANISM(S): Homo sapiens

PROVIDER: GSE236249 | GEO | 2023/12/04

REPOSITORIES: GEO

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Structural basis of nucleosomal H4K20 recognition and methylation by SUV420H1 methyltransferase

Project description:Structural basis of nucleosomal H4K20 recognition and methylation by SUV420H1 methyltransferase

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